中国临床药理学杂志2024，Vol.40Issue(12)：1749-1753,5.DOI:10.13699/j.cnki.1001-6821.2024.12.011
巴马汀抑制人口腔癌KB细胞迁移、侵袭及上皮间充质转化的研究
Effect of palmatine inhibiting migration,invasion and epithelial mesenchymal transformation in human oral cancer KB cells
摘要
Abstract
Objective To investigate the effects of palmatine on migration,invasion and epithelial mesenchymal transformation(EMT)in human oral cancer KB cells.Methods KB cells were divided into control group and palmatine-L,-M,-H groups,cultured with 0,4,8 and 16 μmol·L-1 palmatine.After incubation for 48 h,scratch assay was used to detect migration;Traswell assay was used to detect invasion;matrix metalloproteinase 2(MMP-2),MMP-9 and fibronectin(FN)contents were detected by enzyme-linked immunosorbent assay;the expression of Vimentin,N-cadherin and E-cadherin mRNA were detected by real-time quantitative polymerase chain reaction;the expression of Vimentin,N-cadherin,E-cadherin,Wnt3 and β-catenin protein were detected by Western blot.Results Cell mobility in control group and palmatine-L,-M,-H groups were(69.27±8.62)％,(52.94±4.49)％,(45.22±5.05)％and(37.63±4.88)％;the number of transmembrane cells were 197.33±20.26,125.33±12.01,97.00±9.17 and 62.67±7.51;the content of MMP-2 were(2.93±0.21),(1.49±0.13),(1.16±0.15)and(0.95±0.09)ng·mL-1;the content of MMP-9 were(3.51±0.36),(2.37±0.23),(2.06±0.35)and(1.72±0.16)ng·mL-1;the content of FN were(41.28±4.02),(24.03±3.17),(20.67±2.63)and(13.82±2.19)ng·mL-1;the above indexes in palmatine-L,-M,-H groups were compared with the control group,and the differences were statistically significant(P＜0.05,P＜0.01).The mRNA and protein expressions of Vimentin,N-cadherin and E-cadherin,and the expressions of Wnt3 and β-catenin protein in palmatine-L,-M,-H groups were statistically significant compared with those in control group(P＜0.05,P＜0.01).Conclusion Palmatine can inhibit the migration,invasion and EMT of human oral cancer KB cells,and its mechanism is related to the regulation of Wnt/β-catenin signaling pathway.关键词
巴马汀/口腔癌/上皮间充质转化/迁移/侵袭Key words
palmatine/oral cancer/epithelial mesenchymal transformation/migration/invasion分类
医药卫生引用本文复制引用
程学云,胡光耀,刘红丽,刘晨光,丁园李,杨惠宁,赵一安,孙志广..巴马汀抑制人口腔癌KB细胞迁移、侵袭及上皮间充质转化的研究[J].中国临床药理学杂志,2024,40(12):1749-1753,5.基金项目
吉林省教育厅科学研究基金资助项目(JJKH20230084KJ) （JJKH20230084KJ）
吉林省卫生健康科技能力提升基金资助项目(2023JC056) （2023JC056）
