中国乳业Issue(6)：80-84,5.DOI:10.12377/1671-4393.24.06.15
生乳中抗生素测定值的不确定度测定
Determination of Uncertainty in the Determination of Antibiotics in Raw Milk
陈炜楠 1杨爱君 1何瑛 1梁俊健 1曹学思1
作者信息
- 1. 广东燕塘乳业股份有限公司,广东广州 510000
- 折叠
摘要
Abstract
[Objective]To evaluate the uncertainty of the SNAP dual-flow enzyme-linked immunosorbent assay(ELISA)for determining the antibiotic content in raw milk.[Methods]Using a three-in-one test strip,antibiotics such as β-lactam,tetracycline,and cephalexin in raw milk were measured.The sources of uncertainty were analyzed,and a complete mathematical model was established.Each component in the model was analyzed and evaluated.The relative standard uncertainty was synthesized and expanded to obtain the expanded uncertainty of the antibiotic measurements for β-lactam,tetracycline,and cephalexin in raw milk samples.[Results]When the confidence level was 95%,the results of the antibiotic measurements in raw milk were:X(β-lactam)=0.64±0.173 8,with k=2,and the expanded uncertainty was U=0.173 8.X(tetracycline)=0.62±0.168 4,with k=2,and the expanded uncertainty was U=0.168 4.X(cephalexin)=0.37±0.100 4,with k=2,and the expanded uncertainty was U=0.100 4 mg/kg.[Conclusion]The uncertainty in the experimental process mainly originates from sample repeatability,sampling volume,test strip reader,and result calculation.This method can provide a reference for evaluating the uncertainty of the SNAP dual-flow ELISA for determining antibiotic levels in raw milk.关键词
SNAP双流向酶联免疫法/生乳/抗生素/测定值/不确定度Key words
SNAP two-flow enzyme immunoassay/raw milk/antibiotics/determination/uncertainty引用本文复制引用
陈炜楠,杨爱君,何瑛,梁俊健,曹学思..生乳中抗生素测定值的不确定度测定[J].中国乳业,2024,(6):80-84,5.
