中国兽医科学2024，Vol.54Issue(6)：711-717,7.DOI:10.16656/j.issn.1673-4696.2024.0100
HA蛋白S227N突变对基于新城疫病毒载体的H7N9亚型禽流感疫苗免疫原性的影响
Effect of the S227N substitution in the hemagglutinin on the immunogenicity of Newcastle disease virus vectored-H7N9 avian influenza vaccine
摘要
Abstract
The purpose of this study is to evaluate the effect of the S227N substitution in the hemagglutinin(HA)on the immunogenicity of Newcastle disease virus(NDV)-vectored H7N9 avian influenza vaccine in chickens.The recombinant NDV expressing the HA with the S227N substitution was generated using reverse genetics and its biological characteristics and immunogenicity in chickens were as-sessed.The results indicated that the recombinant NDV expressing the HAS227N had low virulence(MDT＞120 h),high virus titer(HA titer∶512;EID50∶109.3/mL)and replication capacity in chicken embryonated eggs.These features were comparable to those of the vector virus and the recombinant NDV expressing the wild-type HA protein.The S227N substitution did not change HA expression pattern in the cells inocu-lated with the recombinant NDVs.Additionally,the recombinant NDVs expressing the HAS227N or the wild-type HA protein induced high hemagglutination-inhibition(HI)antibody titers against NDV.The HA S227N substitution increased seroconversion of chickens immunized with the recombinant NDV,whereas it failed to significantly elevate HI titers against H7N9 virus.In conclusion,the S227N substitution in HA cannot significantly enhance the immunogenicity of NDV-vectored H7N9 vaccine in chickens.This study presented helpful information for the design,optimization and evaluation of H7N9 avian influen-za vaccines.关键词
H7N9亚型禽流感病毒/疫苗/新城疫病毒载体/HA蛋白/S227N突变/免疫原性Key words
H7N9 subtype avian influenza virus/vectored-vaccine/Newcastle disease virus/HA protein/S227N substitution/immunogenicity分类
畜牧业引用本文复制引用
傅彦贇,石磊,杨星竹,胡娇,胡增垒..HA蛋白S227N突变对基于新城疫病毒载体的H7N9亚型禽流感疫苗免疫原性的影响[J].中国兽医科学,2024,54(6):711-717,7.基金项目
现代农业产业技术体系专项(CARS-40) （CARS-40）
扬州市重点研发(现代农业)计划项目(YZ2022052) （现代农业）
扬州大学高端人才支持计划项目 （）
扬州大学2023年度大学生学术科技创新基金项目(XCX20230756) （XCX20230756）
