中国兽医科学2024，Vol.54Issue(6)：749-756,8.DOI:10.16656/j.issn.1673-4696.2024.0109
鹅星状病毒与新型鸭呼肠孤病毒双重RT-PCR检测方法的建立及应用
Establishment and application of double RT-PCR detection method for goose astrovirus and novel duck reovirus
摘要
Abstract
In this study,specific primers were designed based on the conserved sequence of goose astrovirus(GAstV)ORFlb gene and novel duck reovirus(NDRV) σ C gene,and a dual RT-PCR method of GAstV and NDRV was established.This method has high specificity to amplify fragments of 903 bp(GAstV)and 226 bp(NDRV).The minimum sensitivity of this method was 1.0 × 103 copies/μL.In 320 samples randomly taken from goose farms of different scales in the central,northwestern,and southern regions of Shandong,the positive rate of GAstV was 21.56％(69/320),the positive rate of NDRV was 3.44％(11/320),and the posi-tive rate of mixed infection was 2.81％(9/320).The results of the single RT-PCR were consistent with the dual method.This provides a reliable basis for the rapid diagnosis of GAstV and NDRV.关键词
鹅星状病毒/鹅源新型鸭呼肠孤病毒/双重RT-PCRKey words
goose astrovirus/novel duck reovirus/dual RT-PCR分类
农业科技引用本文复制引用
张景辉,郭慧君,邱建华,亓丽红,宋玲玲,武景琦,房立春,刘涛,吴家强,于可响,徐怀英,吕俊峰,艾武..鹅星状病毒与新型鸭呼肠孤病毒双重RT-PCR检测方法的建立及应用[J].中国兽医科学,2024,54(6):749-756,8.基金项目
中央引导地方科技发展资金项目(YDZX2023009) （YDZX2023009）
山东省重点研发计划(竞争性创新平台)项目(2022CXPT022) （竞争性创新平台）
山东省科技型中小企业创新能力提升工程项目(2022TSGC2342) （2022TSGC2342）
