鹅星状病毒与新型鸭呼肠孤病毒双重RT-PCR检测方法的建立及应用OA北大核心CSTPCD
Establishment and application of double RT-PCR detection method for goose astrovirus and novel duck reovirus
根据鹅星状病毒(GAstV)ORF1b基因与鹅源新型鸭呼肠孤病毒(NDRV)σC基因的保守序列设计特异性引物,建立GAstV与NDRV双重RT-PCR检测方法,该方法具有较高的特异性,在903 bp(GAstV)和226 bp(NDRV)可检测到特异性条带,最低灵敏度为1.0× 103copies/μL.在鲁中、鲁西北及鲁南地区不同规模的养鹅场中随机采取320份样本,GAstV的阳性率为21.56％(69/320),NDRV的阳性率为3.44％(11/320),混合感染阳性率为2.81％(9/320),单一 RT-PCR结果与双重RT-PCR方法一致.该方法的建立为快速诊断GAstV和NDRV提供了可靠的手段.
In this study,specific primers were designed based on the conserved sequence of goose astrovirus(GAstV)ORFlb gene and novel duck reovirus(NDRV) σ C gene,and a dual RT-PCR method of GAstV and NDRV was established.This method has high specificity to amplify fragments of 903 bp(GAstV)and 226 bp(NDRV).The minimum sensitivity of this method was 1.0 × 103 copies/μL.In 320 samples randomly taken from goose farms of different scales in the central,northwestern,and southern regions of Shandong,the positive rate of GAstV was 21.56％(69/320),the positive rate of NDRV was 3.44％(11/320),and the posi-tive rate of mixed infection was 2.81％(9/320).The results of the single RT-PCR were consistent with the dual method.This provides a reliable basis for the rapid diagnosis of GAstV and NDRV.
张景辉;郭慧君;邱建华;亓丽红;宋玲玲;武景琦;房立春;刘涛;吴家强;于可响;徐怀英;吕俊峰;艾武
山东省农业科学院畜牧兽医研究所,山东济南 250131||山东农业大学动物科技学院,山东泰安 271017山东农业大学动物科技学院,山东泰安 271017山东省农业科学院畜牧兽医研究所,山东济南 250131山东省农业科学院家禽研究所,山东济南 250131
畜牧业
鹅星状病毒鹅源新型鸭呼肠孤病毒双重RT-PCR
goose astrovirusnovel duck reovirusdual RT-PCR
《中国兽医科学》 2024 (006)
749-756 / 8
中央引导地方科技发展资金项目(YDZX2023009);山东省重点研发计划(竞争性创新平台)项目(2022CXPT022);山东省科技型中小企业创新能力提升工程项目(2022TSGC2342)
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