中国体外循环杂志2024，Vol.22Issue(3)：232-236,5.DOI:10.13498/j.cnki.chin.j.ecc.2024.03.13
体外循环变温水箱非结核分枝杆菌培养及鉴定研究
Detection and identification of non-tuberculous mycobacterium in heater-cooler units used in cardiopulmonary bypass
闫姝洁 1吉冰洋 1陶随 1郑伟 1杨林 1亢一舟 1王梦珍 2李明 2薄致远 1窦红涛2
作者信息
- 1. 100037 北京,中国医学科学院阜外医院体外循环科
- 2. 100730 北京,北京协和医学院北京协和医院检验科
- 折叠
摘要
Abstract
Objective This study aimed to investigate the situation of non-tuberculous mycobacterium(NTM)contamination in heater-cooler unit(HCU)used in cardiopulmonary bypass.Methods Water samples were collected from the nineteen HCU in our hospital and sent for NTM culture.Furtherly,NTM-positvie heater-cooler devices were sampled in water tank biofilm and air outlet for NTM identification via sequencing two DNA regions in rpoβ and 16S rRNA.Results Water samples from four HCU were tested NTM positive.In two HCUs,M.chimaera were detected in both the water tank biofilms and air outlets.In the third HCU,M.chimaera was detected only in water tank biofilm.In the last HCU,M.chimaera and M.gordonae were detected in water tank biofilm and air outlet,respectively.Conclusion Several HCUs in our hospital were contaminated with NTM(mostly with M.chimera).Measure should be taken to reduce the risks of NTM contamination and HCU-related NTM infection:frequent NTM monitoring,using filtered tap water,scheduled biofilm removal,optimal HCU disinfection protocol,and placing HCU's exhaust flow away from the operating field.关键词
变温水箱/奇美拉分枝杆菌/非结核分枝杆菌/院内感染Key words
Heater-cooler unit/Mycobacterium chimaera/Non-tuberculous mycobacterium/Nosocomial infection引用本文复制引用
闫姝洁,吉冰洋,陶随,郑伟,杨林,亢一舟,王梦珍,李明,薄致远,窦红涛..体外循环变温水箱非结核分枝杆菌培养及鉴定研究[J].中国体外循环杂志,2024,22(3):232-236,5.
