中国兽医科学2024，Vol.54Issue(6)：793-800,8.DOI:10.16656/j.issn.1673-4696.2024.0092
邓氏巴贝斯虫血清学诊断抗原的筛选
Screening of serological diagnostic antigens for Babesia duncani infection
摘要
Abstract
In order to identify potential diagnostic antigens for detecting Babesia duncani infection,exoantigens(ExoAgs)secreted into host sera were screened by quantitative proteomics,and bioinformatics analysis,molecular biology techniques,and serological reactions were used to analyze and validate their potential as diagnostic antigens.The results showed that a total of 34 ExoAgs were identified using proteomics techniques.Bioinformatics analysis revealed that 8 ExoAgs did not have homologous proteins in Apicomplexan parasites,thus,these 8 ExoAgs were regarded as B.duncani species-specific antigens.Further analysis found that 6 ExoAgs were successfully expressed in eukaryotic cells,and 2 proteins(ExoAg-1200 and ExoAg-2451)could react specifically with B.duncani positive sera,but no reaction with healthy hamster sera.These results indicate that 2 B.duncani species-specific antigens were obtained in this study,and our research may provide biomaterials for the establishment of B.duncani serological detection methods in the future.关键词
邓氏巴贝斯虫/巴贝斯虫病/分泌性抗原/蛋白质组学/诊断抗原Key words
Babesia duncani/babesiosis/exoantigens/proteomics/diagnostic antigen分类
农业科技引用本文复制引用
年月丽,王锦明,张尚弟,张卫军,关贵全,殷宏,尤崇革..邓氏巴贝斯虫血清学诊断抗原的筛选[J].中国兽医科学,2024,54(6):793-800,8.基金项目
十四五国家重点研发计划项目(2022YFD1800200) （2022YFD1800200）
国家自然科学基金项目(31972701) （31972701）
国家寄生虫资源库项目(NPRC-2019-194-30) （NPRC-2019-194-30）
国家肉牛牦牛产业技术体系(CARS-37) （CARS-37）
甘肃省自然科学基金项目(22JR5RA031) （22JR5RA031）
甘肃省基础研究创新群体项目(22JR5RA024) （22JR5RA024）
甘肃省科技厅特派团项目(22CX8NA011) （22CX8NA011）
甘肃省自然科学基金项目(23JRRA1632) （23JRRA1632）
全国实验室重大成果培育项目(SKLADCP2023HP04) （SKLADCP2023HP04）
农业科技创新工程项目(ASTIP) （ASTIP）
