中国兽医科学2024，Vol.54Issue(6)：801-807,7.DOI:10.16656/j.issn.1673-4696.2024.0108
长角血蜱Septin-3的原核表达及其免疫分析
Prokaryotic expression and immune analysis of Septin-3 in Haemaphysalis longicornis(Acari:Ixodidae)
摘要
Abstract
To investigate the structure,biological function and immunological properties of Septin-3 protein of Haemaphysalis longicornis,molecular structure of Septin-3 protein was resolved by gene cloning and prokaryotic protein expression,the immune properties were analyzed using the preparation of polyclonal antibodies,ELISA for antibody potency and Western-blot detection,and Septin-3 protein function was validated in conjunction with animal infection experiment.The results showed that the target fragment size of Septin-3 gene was 1 017 bp,and the Septin-3 gene can encode about 338 amino acid residues,and it can express a protein of 38 ku,the antibody potency of the Septin-3 protein was as high as 1∶256 000.The study conducted tick attack experiments on New Zealand white rabbits,compared with the control group,the results showed that H.longicornis,its days of saturation increased by 1.34 d,its weight after saturation decreased by 20.03-24.75 mg,and its saturation rate decreased by 11.04％.The research results suggest that the recombinant Septin-3 protein has excellent immunogenicity and reactivity,and has a certain inhibitory effect on the growth and development of H.longicornis.关键词
长角血蜱/Septin-3基因/原核表达/抗体/免疫分析Key words
Haemaphysalis longicornis/Septin-3 gene/prokaryotic expression/antibody/immune analysis分类
农业科技引用本文复制引用
车丽妍,张文霞,罗金,齐萌,王彦龙,张振杰,刘光远,关贵全,殷宏,任巧云,赵爱云,王国辉..长角血蜱Septin-3的原核表达及其免疫分析[J].中国兽医科学,2024,54(6):801-807,7.基金项目
国家寄生虫资源中心项目(2019-194-30) （2019-194-30）
中国农业科学院科技创新工程项目(ASTIP) （ASTIP）
甘肃省基础研究创新群体项目(22JR5RA024) （22JR5RA024）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
家畜疫病病原生物学国家重点实验室开放基金课题(SKLVEB2021KFKT012) （SKLVEB2021KFKT012）
