中国兽医科学2024，Vol.54Issue(6)：816-823,8.DOI:10.16656/j.issn.1673-4696.2024.0114
猪繁殖与呼吸综合征病毒作为外源基因表达活载体的研究进展
Research advance in porcine reproductive and respiratory syndrome virus as a live vector to express foreign genes
摘要
Abstract
Since the first isolation of porcine reproductive and respiratory syndrome virus(PRRSV)in the United States(1987)and Europe(1990),the virus has been circulating globally for over 30 years.Until today,PRRSV continues to mutate and evolve,and its epidemic situation in China is particu-larly complex.In the post African swine fever era,it remains one of the most economically significant pathogens affecting the pig farming industry.Currently,commercially available vaccines include tra-ditional inactivated virus vaccines(KV)and modified live virus vaccines(MLV).However,vaccine preven-tion and control of PRRSV have not yet been fully implemented.There is an urgent need to develop a safe,effective,and universal genetic engineering vaccine.Based on PRRSV infectious cloning reverse genet-ic platform,the expression of foreign genes,including marker genes,immune enhancing genes,or other viral antigen genes,has become a research hotspot.The construction of recombinant vaccines,gene dele-tion vaccines,and broad-spectrum universal vaccines is needed.Herein,we review the research advance of PRRSV as a live vector for expressing foreign genes and its potential application prospects in the development of new genetic engineering vaccines in recent years.关键词
猪繁殖与呼吸综合征病毒(PRRSV)/基因工程疫苗/感染性克隆/外源基因Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/genetic engineering vac-cines/infectious clone/foreign gene分类
农业科技引用本文复制引用
王树茂,王桂花,王安冉,段宏勇,姜一峰,周艳君,高飞,李丽薇..猪繁殖与呼吸综合征病毒作为外源基因表达活载体的研究进展[J].中国兽医科学,2024,54(6):816-823,8.基金项目
国家重点研发计划项目(2022YFD1800305,2022YFD1800804,2021YFD1801401) （2022YFD1800305,2022YFD1800804,2021YFD1801401）
上海市科技兴农项目(2022-02-08-00-12-F01162) （2022-02-08-00-12-F01162）
中国农业科学院科技创新工程项目(CAAS-CSLPDCP-202301) （CAAS-CSLPDCP-202301）
