中国药理学与毒理学杂志2024，Vol.38Issue(6)：445-454,10.DOI:10.3867/j.issn.1000-3002.2024.06.006
Anoctamin家族中钙激活氯离子通道调节剂筛选及应用研究进展
Research progress in screening and applications of calcium-activated chloride ion channel modulators in Anoctamin family
摘要
Abstract
Calcium-activated chloride channels(CaCCs)are a class of channel proteins that trans-port chloride ions activated by intracellular calcium,which play a crucial role in regulating membrane potential,intracellular calcium balance,and cell excitability,particularly in neurons and muscle cells.In the Anoctamin(Ano)family,Ano1 is the most classic CaCC.Targeted modulators of Ano1 have poten-tial therapeutic effects against such diseases as cancer,cystic fibrosis,hypertension,diarrhea,and asthma.Since the discovery of Ano1 in 2008,several methods for screening CaCC-specific modulators have emerged including high-throughput primary screening of fluorescent proteins,electrophysiological patch clamp technique and virtual screening,and identification of small molecule modulators with diverse pharmacological effects.This paper summarizes the principles,advantages and disadvantages of the mainstream screening methods,and reviews the chemical structures and potential applications of Ano1-specific modulators discovered to date.关键词
钙激活氯离子通道/Anoctamin/Ano1/Ano2/高通量筛选/特异性调节剂Key words
calcium-activated chloride channels/Aonctamin/Ano1/Ano2/high-throughput screening/specific modulators分类
医药卫生引用本文复制引用
吴明达,洪啟元,兰月娇,姚岚,习诗婷,刘雪莹,高俊涛,郑锴,郝峰..Anoctamin家族中钙激活氯离子通道调节剂筛选及应用研究进展[J].中国药理学与毒理学杂志,2024,38(6):445-454,10.基金项目
吉林省科技发展计划项目(20220101338JC) （20220101338JC）
吉林省教育厅课题(JJKH20220462KJ) （JJKH20220462KJ）
吉林省教育厅课题(JJKH20220473SK) （JJKH20220473SK）
吉林省大学生创新创业项目(S202213706026) （S202213706026）
吉林省大学生创新创业项目(S202213706019) Jilin Science and Technology Development Program Project(20220101338JC) （S202213706019）
Project of Jilin Provincial Department of Education(JJKH20220462KJ) （JJKH20220462KJ）
Project of Jilin Provincial Department of Education(JJKH20220473SK) （JJKH20220473SK）
Jilin Province University Students Innovative Entrepreneurship Project(S202213706026) （S202213706026）
and Jilin Province University Students Innovative Entrepreneurship Project(S202213706019) （S202213706019）
