中国农业科学2024，Vol.57Issue(11)：2079-2091,13.DOI:10.3864/j.issn.0578-1752.2024.11.003
大豆地方种质资源鲜籽粒可溶性糖含量的全基因组关联分析
Genome Wide Association Analysis of Soluble Sugar Content in Fresh Seeds of Soybean Landraces
摘要
Abstract
[Objective]Soluble sugar content is one of the important quality traits of vegetable soybean.The genetic variation and genetic mechanism of soluble sugar content in fresh soybean seeds were studied to provide a basis for germplasm innovation and quality breeding of vegetable soybean.[Method]Using 133 soybean landraces from the Northeast region,the North region,the Huanghuaihai region and the South region,the soluble sugar content of fresh seeds was determined in the three environments of Lianjiang in spring,Fuqing in spring and autumn,in 2021.In combination with 82 187 high-quality SNP markers,the whole genome association analysis of soluble sugar content was conducted based on the mixed linear model MLM(Q+K),and the SNP loci with significantly related to soluble sugar content were identified.The candidate intervals were selected by the significant SNP loci and the extension of 119.07 kb linkage disequilibrium decay distance at both ends.The candidate genes were predicted according to the annotation and tissue expression information of the genes in the candidate intervals.[Result]The variation range of soluble sugar content in fresh seeds under three environments was 3.37-33.84 mg·g-1,the genetic variation coefficient was 24.59％-32.69％,and the heritability of soluble sugar content was 68.14％.6,8 and 22 SNPs were significantly associated with the soluble sugar content of fresh seeds were detected in Lianjiang in spring,Fuqing in spring and autumn,respectively,and phenotypic variation was 12.43％-29.27％.A total of 86 genes were obtained in the candidate regions of 9 significant SNP loci with higher interpretation rate of phenotypic variation,and 9 candidate genes were further screened by gene annotation and tissue expression information.These candidate genes are mainly involved in biological processes such as transcription factors,glycoprotein families and carbohydrate synthesis and transport.Among them,Glyma.01g016500,Glyma.13g042100,Glyma.16g131800 and Glyma.16g155300 were more highly expressed in soybean seeds and pods,which can be used as the most potential candidate genes for soluble sugar in fresh soybean seeds.[Conclusion]Through genome-wide association analysis,36 SNPs significantly associated with soluble sugar content in fresh seeds were detected,and 9 candidate genes were further screened out,which may be involved in the regulation of soluble sugar content in fresh soybean seeds.Among them,Glyma.01g016500,Glyma.13g042100,Glyma.16g131800 and Glyma.16g155300 can be the key candidate genes for regulating soluble sugar content in fresh soybean seeds.关键词
大豆/鲜籽粒/可溶性糖含量/全基因组关联分析/候选基因Key words
soybean/fresh seeds/soluble sugar contents/genome wide association analysis/candidate genes引用本文复制引用
张玉梅,丁文涛,蓝新隆,李清华,胡润芳,郭娜,林国强,赵晋铭..大豆地方种质资源鲜籽粒可溶性糖含量的全基因组关联分析[J].中国农业科学,2024,57(11):2079-2091,13.基金项目
国家重点研发计划(2021YFD1201605)、福建省自然科学基金(2020J011372)、福建省属公益类科研院所基本科研专项(2022R1031006)、福建省财政专项(FJVRC2020-02、CXTD2021011-2) （2021YFD1201605）
