中国医学前沿杂志（电子版）2024，Vol.16Issue(5)：50-58,9.DOI:10.12037/YXQY.2024.05-09
中药复方金思维含药血清调控PI3K/AKT/GSK-3β信号通路对拟阿尔茨海默病细胞模型tau蛋白磷酸化的影响
Effects of traditional Chinese medicine compound GAPT serum on regulation of PI3K/AKT/GSK-3β signaling pathway through P-tau in Alzheimer disease cell models
摘要
Abstract
Objective To investigate the effect and mechanism of traditional Chinese medicine compound GAPT serum on regulation of P-tau in Alzheimer disease(AD)cell models.Methods Aβ25-35 induced human neuroblastoma cells(SH-SY5Y)were used to establish an AD cell model,and the optimal concentration of PI3K/AKT/GSK-3β signaling pathway inhibitor LY294002 and GAPT serum were screened by CCK-8.SH-SY5Y cells were divided into normal group,model group,GAPT low-dose group,GAPT medium-dose group,GAPT high-dose group and LY294002 group.The effect of GAPT serum on tau protein phosphorylation and PI3K/AKT/GSK-3β pathway related proteins in SH-SY5Y cells was detected by western blot.Results SH-SY5Y cells were treated with 20 μmol/L Aβ25-35 for 24 h to establish the best AD cell model.Compared with the normal group,P-AKT was significantly reduced(P＜0.05)and P-tau was significantly increased(P＜0.01).Compared with the model group,the expression of PI3K(P＜0.05)was significantly increased in the GAPT low-dose group,the expression of GSK-3β(P＜0.05)in the GAPT high-dose group,and the expression of P-tau(P＜0.01)in the GAPT medium-dose and high-dose group(P＜0.01)(P＜0.001).After the addition of pathway inhibitor LY294002,the expression of P-PI3K,PI3K,P-AKT,AKT,GSK-3β and P-tau was not significantly different from that in the model group(P＞0.05).Conclusions GAPT serum can reduce the expression of P-tau in AD cell models,and the mechanism may be related to the activation of PI3K/AKT/GSK-3β signaling pathway.关键词
阿尔茨海默病/金思维/PI3K/AKT/GSK-3β信号通路/tau蛋白磷酸化Key words
Alzheimer disease/GAPT/PI3K/AKT/GSK-3β signaling pathway/P-tau引用本文复制引用
马澜,时晶,李婷,孙庆玲,魏明清,倪敬年..中药复方金思维含药血清调控PI3K/AKT/GSK-3β信号通路对拟阿尔茨海默病细胞模型tau蛋白磷酸化的影响[J].中国医学前沿杂志（电子版）,2024,16(5):50-58,9.基金项目
National Natural Science Foundation of China(82074362) （82074362）
"Decoding Traditional Chinese Medicine"Key Collaborative Project of Beijing University of Chinese Medicine(BZY-JMZY-2022-002) 国家自然科学基金(82074362) （BZY-JMZY-2022-002）
北京中医药大学"解码中医"协同攻关项目(BZY-JMZY-2022-002) （BZY-JMZY-2022-002）
