中药复方金思维含药血清调控PI3K/AKT/GSK-3β信号通路对拟阿尔茨海默病细胞模型tau蛋白磷酸化的影响OA北大核心CSTPCD
Effects of traditional Chinese medicine compound GAPT serum on regulation of PI3K/AKT/GSK-3β signaling pathway through P-tau in Alzheimer disease cell models
目的 探讨中药复方金思维含药血清对拟阿尔茨海默病(Alzheimer disease,AD)细胞模型tau蛋白磷酸化的影响及作用机制.方法 采用Aβ25-35诱导人神经母细胞瘤细胞(SH-SY5Y)建立拟AD细胞模型,CCK-8法筛选金思维含药血清、PI3K/AKT/GSK-3β通路抑制剂LY294002最佳作用浓度.将SH-SY5Y细胞分为正常组、模型组、金思维低剂量组、金思维中剂量组、金思维高剂量组以及LY294002组.蛋白免疫印迹法检测金…查看全部>>
Objective To investigate the effect and mechanism of traditional Chinese medicine compound GAPT serum on regulation of P-tau in Alzheimer disease(AD)cell models.Methods Aβ25-35 induced human neuroblastoma cells(SH-SY5Y)were used to establish an AD cell model,and the optimal concentration of PI3K/AKT/GSK-3β signaling pathway inhibitor LY294002 and GAPT serum were screened by CCK-8.SH-SY5Y cells were divided into normal group,model group,GAPT low-dose group,GA…查看全部>>
马澜;时晶;李婷;孙庆玲;魏明清;倪敬年
北京中医药大学东直门医院,北京 100700北京中医药大学东直门医院,北京 100700北京中医药大学东直门医院,北京 100700北京中医药大学东直门医院,北京 100700北京中医药大学东直门医院,北京 100700北京中医药大学东直门医院,北京 100700
阿尔茨海默病金思维PI3K/AKT/GSK-3β信号通路tau蛋白磷酸化
Alzheimer diseaseGAPTPI3K/AKT/GSK-3β signaling pathwayP-tau
《中国医学前沿杂志（电子版）》 2024 (5)
50-58,9
National Natural Science Foundation of China(82074362)"Decoding Traditional Chinese Medicine"Key Collaborative Project of Beijing University of Chinese Medicine(BZY-JMZY-2022-002) 国家自然科学基金(82074362)北京中医药大学"解码中医"协同攻关项目(BZY-JMZY-2022-002)
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