棉花-花生轮作下的根系土壤细菌群落结构及功能分析OA北大核心CSTPCD

To enhance the yields of cotton and peanut,bacterial community structure and function in root soil under cotton-peanut rotation were studied.Five treatments were investigated,as continuous cotton cultivation,con-tinuous peanut cultivation,cotton-peanut rotation,peanut-cotton rotation,and fallow land.High-throughput gene sequencing of 16S rRNA was used to analyze bacterial community structure and function.Results revealed that crop rotation increased the diversity of root-associated bacterial communities.A total of 5 009 952 valid sequences were obtained from soil samples,with the highest number of OTUs(operational taxonomic units)under fallow land treat-ment.In rotational cropping systems,Proteobacteria,Acidobacteria,and Actinobacteria were identified as dominant phyla.Additionally,richness of Firmicutes,Bacteroidetes,and Gemmatimonadetes were increased under rotational patterns.Based on different functions,the microbial groups were categorized into 8 classes.Under rotations modes,higher prevalence of functional groups related to microbial genetic inheritance was found.Metabolic-related groups were predominant overall,and significant enrichment of functional protein sets were found associated with human and plant pathogens.These results indicated that cotton-peanut rotation could increase microbial richness and di-versity,alter soil microbial community structures,promote nutrient absorption by crop roots,and ultimately enhance crop yields.