中国免疫学杂志2024，Vol.40Issue(6)：1121-1125,5.DOI:10.3969/j.issn.1000-484X.2024.06.001
不同条件下小鼠肺泡巨噬细胞体外培养的实验研究
Experimental study of mouse alveolar macrophages cultured in vitro under different conditions
摘要
Abstract
Obiective:To compare characteristics of alveolar macrophages(AMs)or AM-like cells in vitro cultured from different progenitors in mice of different ages in presence of GM-CSF,TGF-β and PPAR-γ agonist rosiglitazone(in brief,GTR),according to a previously reported optimized culture procedure.Methods:After bronchoalveolar lavage fluid(BALF)and bone marrow were isolated from mice of different ages and fetal livers were isolated from embryonic-day-15～17 mice,mononuclear cells were seeded into 12-well plates and cultured in presence of GTR.Generated cells were subjected to flow cytometry analysis to analyze expressions of AM markers F4/80,CD11b,CD170 and CD11c.Results:After culture,almost all BALF-derived cells were round-shaped under light microscope,about half bone marrow-derived cells appeared round-shaped,and about 85% fetal liver-derived cells showed round-shaped morphology.Frequency of BALF-AMs from 4-week-old mice was nearly 90%.Frequency and number of BALF-AMs increased with age of mice.Number of BALF-AMs from 12-week-old mice was nearly 1×106.Frequency of bone marrow-derived AM-like cells from 4-week-old mice was lower than 20% and increased to about 40%～45% for 8-week-old and 12-week-old mice.Number of bone marrow-derived AM-like cells increased with age of mice,and about 0.8×106 cells/well bone marrow-derived AM-like cells were produced from 12-week-old mice.Frequency of fetal liver-derived AM-like cells was around 84% and their number could reach 4×106 cells/well.Conclusion:Purity and number of AMs or AM-like cells in vitro cultured from different progenitors in mice of different ages vary dramatically.Detailed condition should be chosen according to specific study requirements.关键词
肺泡巨噬细胞/体外培养/肺泡灌洗液/骨髓/胎肝Key words
Alveolar macrophages/In vitro culture/Bronchoalveolar lavage fluid/Bone marrow/Fetal liver分类
医药卫生引用本文复制引用
云宏芳,蒋慧,牛春晓,张纪岩..不同条件下小鼠肺泡巨噬细胞体外培养的实验研究[J].中国免疫学杂志,2024,40(6):1121-1125,5.基金项目
国家自然科学基金(81930027,92169207). （81930027,92169207）
