中国免疫学杂志2024，Vol.40Issue(6)：1137-1141,5.DOI:10.3969/j.issn.1000-484X.2024.06.004
IFN-β通过STAT1诱导SARI表达抑制AML细胞增殖并促进凋亡
IFN-β inhibits proliferation and promotes apoptosis of AML cells by STAT1-induced SARI
摘要
Abstract
Objective:To investigate effect of SARI expression induced by IFN-β on proliferation and apoptosis of acute myelo-blastic leukemia(AML)cells,and to explore its potential regulatory molecules.Methods:qPCR and Western blot were used to screen AML cells with low SARI expression as experimental cell lines.AML cells were treated with different concentrations of IFN-β,and expression of SARI was detected by qPCR and Western blot at different time to select appropriate concentration and time of IFN-β.RNA-Seq transcriptome sequencing and KEGG enrichment analysis were used to preliminarily screen potential regulatory molecules of IFN-β-induced SARI expression in AML cells.AML cells were treated with corresponding molecular inhibitors combined with IFN-β,cell proliferation was detected by MTS assay,and apoptosis was detected by flow cytometry.To clear this molecule was involved in IFN-β-induced SARI expression on AML cell proliferation and apoptosis.Results:SARI expression in HL60 and NB4 cells were rela-tively decreased,so they were selected as experimental cell lines.After treatment with 1 ng/ml IFN-β for 12 h,SARI expression in AML cells was increased,cell proliferation was inhibited and apoptosis were increased.STAT1 was screened as a potential regulatory mole-cule for IFN-β-induced SARI expression.After inhibiting STAT1,effects of IFN-β on SARI expression,proliferation inhibition and apop-tosis promotion of AML cells were reversed significantly.Conclusion:IFN-β can promote SARI expression in AML cells by STAT1,in-hibit cell proliferation and promote apoptosis.关键词
IFN-β/SARI/STAT1/AML/增殖/凋亡Key words
IFN-β/SARI/STAT1/AML/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
林艳凤,洪小颖,黄莹莹,王小花,吴玮,林东红,薛龑..IFN-β通过STAT1诱导SARI表达抑制AML细胞增殖并促进凋亡[J].中国免疫学杂志,2024,40(6):1137-1141,5.基金项目
福建省自然科学基金面上项目(2021J01812) （2021J01812）
福建省中青年教师教育科研项目(科技类)(JAT190178,JAT190179). （科技类）
