中国免疫学杂志2024，Vol.40Issue(6)：1160-1165,6.DOI:10.3969/j.issn.1000-484X.2024.06.008
ANXA1拟肽Ac2-26对脓毒症大鼠急性肾损伤及中性粒细胞凋亡过程的影响
Effect of ANXA1 peptidomimetic Ac2-26 on acute kidney injury and neutrophil apoptosis in septic rats
摘要
Abstract
Objective:To explore the effect of Annexin A1(ANXA1)peptidomimetic Ac2-26 on acute kidney injury(AKI)and neutrophil apoptosis in septic rats.Methods:Experimental groups included control group,Ac2-26 group,AKI group,AKI+Ac2-26 group,with 15 rats in each group.After cecal ligation and perforation were used to establish a sepsis-induced AKI model,Ac2-26 was intravenously infused for treatment,once a day for 14 days;after the end,ELISA was used to detect levels of serum creatinine(Scr),urea nitrogen(BUN),IL-1β,IL-6 and TNF-α;HE staining and periodic acid Schiff(PAS)staining were used to observe the pathological changes of rat kidney tissues in each group;immunohistochemical staining was used to detect expression of ANXA1 in kidney tissue of each group of rats;neutrophils were isolated from rat peripheral blood,Giemsa staining and trypan blue staining were used to detect cell purity and viability;Annexin V-FITC/PI double staining method and TUNEL staining were used to determine apop-tosis level of neutrophils in each group.Results:Compared with control group,levels of Scr and BUN in serum of rats in AKI group were increased(P<0.05),levels of IL-1β,IL-6 and TNF-α also increased(P<0.05),renal tubules and glomeruli in kidney tissue were both significantly damaged,accompanied by a large number of inflammatory cell infiltration,and pathological score increased(P<0.05),while proportion of ANXA1 positive staining area was decreased(P<0.05);neutrophils identified by Giemsa staining and trypan blue staining had complete morphology and high activity;compared with control group,apoptosis rate of neutrophils in AKI group was decreased(P<0.05),and the positive rate of TUNEL was decreased(P<0.05).Compared with AKI group,levels of Scr and BUN in serum of rats in AKI+Ac2-26 group were decreased(P<0.05),levels of IL-1β,IL-6 and TNF-α also decreased(P<0.05),pathological manifestations of renal tubules and glomeruli in renal tissue were significantly reduced,and pathological score was reduced(P<0.05),while the proportion of ANXA1 positive staining area was increased(P<0.05),at the same time,apoptosis rate of rat neu-trophils was increased(P<0.05),positive rate of TUNEL was also increased(P<0.05).Conclusion:ANXA1 peptidomimetic Ac2-26 can increase expression of ANXA1 in kidney tissue of AKI in septic rats,promote neutrophil apoptosis,and have a protective effect on kidney tissue damage in rats caused by sepsis.关键词
脓毒症/急性肾损伤/膜联蛋白A1/中性粒细胞/凋亡Key words
Sepsis/Acute kidney injury/Annexin A1/Neutrophils/Apoptosis分类
医药卫生引用本文复制引用
黄承,蒲运刚,田仁富,杨先芹,张莉..ANXA1拟肽Ac2-26对脓毒症大鼠急性肾损伤及中性粒细胞凋亡过程的影响[J].中国免疫学杂志,2024,40(6):1160-1165,6.基金项目
湖北省卫生健康委2019年度第一批联合基金立项项目(WJ2019H130). （WJ2019H130）
