中国药业2024，Vol.33Issue(12)：62-65,4.DOI:10.3969/j.issn.1006-4931.2024.12.014
人类辅助生殖技术用液中铵离子含量影响因素分析
Analysis of Factors Affecting the Content of Ammonium Ions in Fluids for Assisted Reproductive Technology
管瑞芹 1赵丹妹 2姜爱莉 3柯林楠 2王召旭2
作者信息
- 1. 中国食品药品检定研究院,北京 102629||烟台大学,山东 烟台 264005
- 2. 中国食品药品检定研究院,北京 102629
- 3. 烟台大学,山东 烟台 264005
- 折叠
摘要
Abstract
Objective To provide a reference for the control of ammonium ion content and storage of fluids for assisted reproductive technology(ART).Methods Seventeen batches of ART fluids with different functions from six manufacturers on the market were selected,the ion chromatography(IC)method was used to determine the ammonium ion content,and the effects of storage temperature and storage time on the ammonium ion content were analyzed.Results During the shelf-life,the ammonium ion contents of refrigerant kit,thawing fluid kit,medium and handling fluids were in the range of 0.89 to 3.34 μg/mL,0.88 to 4.68 μg/mL,0.75 to 1.45 μg/mL,0.38 to 3.89 μg/mL respectively.The content of ammonium ions in embryo culture fluids increased from 0.9 μg/mL to 3.1 μg/mL stored at 37℃within 30 d,and from 0.6 μg/mL to 1.7 μg/mL stored at 4℃within 240 d.Conclusion The contents of ammonium ions in ART fluids with different functions are diverse,and they are positively correlated with the storage temperature and storage time of the products.The ammonium ion content in the products should be considered when formulating the shelf-life.关键词
人类辅助生殖技术/液体类产品/离子色谱法/铵离子/含量测定/质量控制/储存温度/储存时间Key words
assisted reproductive technology/fluid product/ion chromatography/ammonium ion/content determination/quality control/storage temperature/storage time分类
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管瑞芹,赵丹妹,姜爱莉,柯林楠,王召旭..人类辅助生殖技术用液中铵离子含量影响因素分析[J].中国药业,2024,33(12):62-65,4.
