肿瘤防治研究2024，Vol.51Issue(6)：440-447,8.DOI:10.3971/j.issn.1000-8578.2024.23.1034
AAMP通过调节YAP信号通路促进骨肉瘤细胞转移的机制研究
AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway
摘要
Abstract
Objective To determine the role of AAMP in osteosarcoma cells and explore the mechanism of invasion and metastasis of osteosarcoma cells regulated by AAMP through the YAP signaling pathway.Methods Public sequencing data analysis was used to explore the correlation between AAMP and osteosarcoma.q-PCR,Western blot,and immunohistochemistry were used to detect the expression levels of osteosarcoma cell-related molecules.CCK-8 was used to detect cell proliferation ability.Transwell and wound healing assays were used to detect the invasive and metastatic abilities of osteosarcoma cells.Immunofluorescence was used to detect the cell localization and expression levels of related molecules.Results High expression of AAMP was negatively correlated with the prognosis of patients with osteosarcoma(P<0.05),and the expression of AAMP in patients with metastatic osteosarcoma increased(P<0.05).Compared with the control group,the AAMP interference group showed significantly decreased migratory,invasive,and EMT activities(P<0.05).The expression of p-CFL1 reduced after the knockdown of AAMP,and the cell plate pseudopods decreased significantly(P<0.05).A positive correlation was found between the expression levels of AAMP and YAP in osteosarcoma cells(P<0.05).Interfering with YAP expression can affect the migration and invasion of osteosarcoma cells.Conclusion AAMP promotes osteosarcoma cell metastasis by regulating the YAP signaling pathway,suggesting that AAMP may be a key molecule in promoting invasion and metastasis of osteosarcoma.关键词
骨肉瘤/AAMP/YAP/丝切蛋白1/转移/侵袭/伪足Key words
Osteosarcoma/AAMP/Y-AP/CFL1/Migration/Invasion/Pseud-opodium分类
医药卫生引用本文复制引用
邓乾蓉,占方彪,谢朝政,向双,陈建,杨毅..AAMP通过调节YAP信号通路促进骨肉瘤细胞转移的机制研究[J].肿瘤防治研究,2024,51(6):440-447,8.基金项目
重庆市自然科学基金面上项目(cstc2021jcyjmsxmX0909) （cstc2021jcyjmsxmX0909）
重庆市中青年医学高端人才项目(33158) （33158）
重庆大学附属三峡医院青年人才重点项目(2022YJKYXM-043),重庆市万州区博士"直通车"科研项目(wzstc20230404) The General Project of Cho-ngqing Natural Science Foundation(No.cstc2021jcyjmsxmX0909) （2022YJKYXM-043）
The Young and Middle-aged Medical High-end Ta-lent Project of Chongqing(No.33158) （No.33158）
The Key Project for Young Talents of Chongqing University Three Gorges Hospital(No.2022YJKYXM-043) （No.2022YJKYXM-043）
The Wanzhou District of Chongqing PhD Zhitongche Research Project(No.wzs-tc20230404) （No.wzs-tc20230404）
