中国现代中药2024，Vol.26Issue(6)：989-996,8.DOI:10.13313/j.issn.1673-4890.20231031001
基于指标成分及指纹图谱多模式识别的不同品种红花质量评价
Quality Evaluation of Different Varieties of Safflower Based on Index Components and Multiple Pattern Recognition Method of Fingerprint
摘要
Abstract
Objective:The index components of different varieties of safflower were determined,and fingerprints were established.Their quality was evaluated by the chemical recognition pattern method,and a method for comparing the quality of different varieties of safflower was established.Methods:The contents of hydroxyl safflower yellow A(HSYA)and kaempferol in 32 batches of different varieties of safflower were determined by high performance liquid chromatography(HPLC).The similarity evaluation system for TCM chromatographic fingerprint(edition 2012)was used to evaluate the similarity of 32 batches of safflower samples.The cluster analysis was carried out by Origin 2022 software,and the principal component analysis was carried out by SIMCA 14.1 software.Results:Only three batches of 32 safflower samples were detected without HSYA and kaempferol.The content of HSYA in the other 29 batches ranged from 0.4%to 2.5%,and the content of kaempferol ranged from 0.004%to 1.060%.A total of 16 common peaks were identified by HPLC fingerprints,and five components,such as tryptophan,HSYA,and 6-hydroxykaempferol-3-O-B-D-glucoside,were identified with similarities ranging from 0.510 to 0.936.The quality of different varieties of safflower was significantly varied.Conclusion:By HPLC content determination,fingerprint establishment,and multi-pattern recognition,the quality differences of different varieties of safflower can be analyzed and compared.The research results provide potential breeding directions for different varieties of safflower and have important reference value for safflower breeding.关键词
红花/指标成分/高效液相色谱法/指纹图谱/化学模式识别/质量评价Key words
safflower/index component/HPLC/fingerprint/chemical pattern recognition/quality evaluation分类
医药卫生引用本文复制引用
苏改兰,李晓瑾,樊丛照,邱远金,张际昭,王果平,赵亚琴,王威,赵连佳,石书兵..基于指标成分及指纹图谱多模式识别的不同品种红花质量评价[J].中国现代中药,2024,26(6):989-996,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
新疆维吾尔自治区天山英才第三期培养人选项目 （）
农业农村部科技发展中心项目 （）
新疆农业科学院青年科技骨干创新能力培养项目(xinkg-2023017) （xinkg-2023017）
