中国现代中药2024，Vol.26Issue(6)：1036-1047,12.DOI:10.13313/j.issn.1673-4890.20240415002
防风对春季低温胁迫的生理响应
Physiological Response of Saposhnikovia divaricate to Cold Stress in Spring
摘要
Abstract
Objective:To investigate the physiological response of Saposhnikovia divaricate to cold stress in spring and determine the best harvest time of S.divaricate according to the content of secondary metabolites.Methods:The fresh roots of S.divaricate were harvested at the end of autumn and in spring,respectively,and the relationships between the reactive oxygen species(ROS)content,antioxidant enzyme activities,key enzyme activity of secondary metabolites,secondary metabolite content,total polysaccharide content,and acid polysaccharide content were investigated.Results:Affected by the low temperatures in spring,the content of ROS and malondialdehyde(MDA),the activities of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD),the activities of key enzymes of secondary metabolites including phenylalanine ammonia-lyase(PAL),acetyl CoA carboxylase(ACC),and chalcone synthase(CHS),and the content of 1,3-disphosphoglycerate(1,3-DPG),a product of the metabolic pathway in the fresh roots of S.divaricate were significantly increased compared with those in autumn.Compared with that in autumn,the content of prim-O-glucosylcimifugin,5-O-methylvisamminol,and cimifugin in spring was increased by 17.7%,36.7%,and 11.1%,respectively,while the content of total polysaccharide and acid polysaccharide in autumn was higher than that in spring.Conclusion:The antioxidant enzymes and secondary metabolites of S.divaricate synergistically maintain the balance of ROS under cold stress in spring,and spring harvest can increase the content of chromone.关键词
防风/采收期/活性氧/次生代谢/色原酮Key words
Saposhnikovia divaricata(Trucz.)Schischk./harvest time/reactive oxygen species/secondary metabolism/chromone分类
医药卫生引用本文复制引用
刘文斐,张炜,王力扬,姚瑶,宋孝文,孟祥才..防风对春季低温胁迫的生理响应[J].中国现代中药,2024,26(6):1036-1047,12.基金项目
国家自然科学基金区域联合项目(U20A20407) （U20A20407）
黑龙江省中医药管理局道地药材标准(团体)制定项目(ZHYCYC2022-003) （团体）
