植物学报2024，Vol.59Issue(3)：383-396,14.DOI:10.11983/CBB24013
蒙古冰草咖啡酸氧甲基转移酶基因AmCOMT1的鉴定及功能分析
Identification and Functional Analysis of an Agropyron mongoli-cum Caffeic Acid 3-O-methyltransferase Gene AmCOMT1
摘要
Abstract
Agropyron mongolicum is one of northern China's most representative perennial forage grasses,showing strong tolerance to cold and drought.In plants,caffeic acid O-methyltransferase(COMT)is a key gene involved in the biosynthesis of lignin and melatonin,and plays an important role in regulating plant growth,biomass quality,and stress tolerance.In this study,through the analysis of the full-length transcriptome data of A.mongolicum,the COMT candidate gene AmCOMT1 was cloned.AmCOMT1 is highly expressed in tissues with high lignin content,such as stem and root,and its expression is induced by a variety of abiotic stresses,including drought and salt.Overexpression of AmCOMT1 in Arabidopsis wild type(Col-0)and mutant(omt1-2)significantly promoted the synthesis of lignin in transgenic Arabidopsis,restoring the lignin content and composition of the mutant to wild type level and the lignin content in Col-0/35S:AmCOMT1 was increased by 11％.In addition,overexpression of AmCOMT1 increased the melatonin content in Col-0/35S:AmCOMT1 transgenic Arabidopsis.Under salt stress conditions,the average root length of this transgenic line increased by 20.3％compared to the wild type,showing higher stress tolerance.In this study,we identified AmCOMT1 from A.mongolicum as a key gene regulating both lignin biosynthesis and melatonin biosynthesis,improving the stress tolerance of transgenic Arabidopsis.Our results highlighted the application potential of AmCOMT1 in genetic improvement of forage grasses through molecular breeding.关键词
蒙古冰草/咖啡酸氧甲基转移酶/木质素/褪黑素/盐胁迫Key words
Agropyron mongolicum/caffeic acid 3-O-methyltransferase/lignin/melatonin/salt stress引用本文复制引用
杜锦瑜,孙震,苏彦龙,王贺萍,刘亚玲,吴振映,何峰,赵彦,付春祥..蒙古冰草咖啡酸氧甲基转移酶基因AmCOMT1的鉴定及功能分析[J].植物学报,2024,59(3):383-396,14.基金项目
内蒙古自治区种业科技创新重大示范工程"揭榜挂帅"项目(No.2022JBGS0014)、中央引导地方科技发展资金(No.2022ZY006)、青岛新能源山东实验室"抓攻关"项目(No.QNESLKPP202302)、国家林业和草原局重大应急科技"揭榜挂帅"项目(No.202201)和呼和浩特市重大科技专项(No.2021-重-社-2) （No.2022JBGS0014）
