植物学报2024，Vol.59Issue(3)：441-451,11.DOI:10.11983/CBB23105
伊藤杂种'和谐'组培快繁体系的建立
Establishment of a Fast Breeding System for Itoh Hybrid'He Xie'in Tissue Culture
摘要
Abstract
An in vitro rapid propagation system using scale buds of Itoh hybrids'He Xie'can overcome the shortcomings of the slow traditional breeding methods and promote the adoption of the excellent Itoh hybrid varieties.In this study,we used the buds of'He Xie'as explants to investigate the effects of various factors including disinfection time,plant growth regulators(PGRs)concentration and root induction time,and different rooted seedling grades on the initiation,prolifera-tion,rooting and domestication of'He Xie'with one-way experimental design.Our results showed:the optimal disinfection time of buds by 2％sodium hypochlorite solution was 12 min,with a contamination rate of 9.09％;the optimal initial culture medium was MS+1.5 mg·L-1 6-BA+0.2 mg·L-1 GA3+0.5 mg·L-1 AgNO3;the optimal proliferation culture medium was MS+450 mg·L-1 CaCl2+0.5 mg·L-1 6-BA+0.2 mg·L-1 IBA+0.2 mg·L-1 GA3+0.5 mg·L-1 AgNO3 with a proliferation rate of 3.3.Rootless seedlings were cultured on root induction medium 1/2MS+1.0 mg·L-1 putrescine+2.0 mg·L-1 IBA for 8 d at 4℃ in dark and then 30 d at room temperature under light,and finally transferred to root formation medium 1/2MS+1.0 g·L-1 AC for 20 d,a rooting rate of 66.7％was observed.The rooted seedlings were transplanted on a growing matrix of per-lite:vermiculite:charcoal soil=1:1:1(v/v/v)for 60 d,with the highest transplant survival rate of 52.0％being observed for first-grade rooted seedlings,but most of the second-and third-grade seedlings being dead,suggesting that the quality of rooting is critical for transplant survival.关键词
伊藤'和谐'/启动与增殖/生根/鳞芽/移栽驯化Key words
Itoh'He Xie'/initiation and proliferation/rooting/scale buds/transplant domestication引用本文复制引用
康敏,张美莹,齐秀双,佟宁宁,李旸,舒庆艳,刘政安,吕长平,彭丽平..伊藤杂种'和谐'组培快繁体系的建立[J].植物学报,2024,59(3):441-451,11.基金项目
国家自然科学基金青年科学基金(No.32201609)和中国科学院植物研究所企业横向项目(No.E0119C6001) （No.32201609）
