安徽大学学报（自然科学版）2024，Vol.48Issue(3)：91-101,11.DOI:10.3969/j.issn.1000-2162.2024.03.013
合成染料与染料脱色过氧化物酶Il-DyP4相互作用的光谱分析
Exploring the interaction between synthetic dyes and dye decolorization peroxidase of Il-DyP4 by spectral analysis
摘要
Abstract
In this work,the authors investigated the interaction between dye-decolorizing peroxidase(Il-DyP4)and dyed substrates using ultraviolet-visible(UV-Vis)absorption spectrum and fluorescence spectrum,which included anthraquinone dyes(reactive blue 19,RB19;reactive blue 4,RB4),azo dyes(reactive purple 5,RV5;reactive black 5,RB5),and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS).The UV-Vis absorption spectrum showed that the substrates studied in this work could form complexes with Il-DyP4.Furthermore,the fluorescence spectrum also showed that the studied dyed substrates had strong fluorescence quenching effect on Il-DyP4,and the type of quenching was static quenching which depended on the complex formation.The apparent binding constants(Ka)of RB19,RB4,RV5,RB5,ABTS interacting with Il-DyP4 were calculated at different temperatures based on the double logarithmic equation as follows:9.386 2×104 L·mol-1(298 K),5.615 7×106 L·mol-1(298 K),1.926 2×106 L·mol-1(288 K),5.277 8×105 L·mol-1(298 K),2.234 6×105 L·mol-1(298 K).According to the analysis of thermodynamic parameters,the interaction between Il-DyP4 and the substrate depended on hydrophobic interaction,which was a spontaneous endothermic process.关键词
染料脱色过氧化物酶/紫外-可见吸收光谱/荧光光谱/染料类底物/疏水相互作用Key words
dye-decolorizing peroxidase/ultraviolet-visible absorption spectrum/fluorescence spectrum/dyed substrate/hydrophobic interaction分类
生物科学引用本文复制引用
严凤燕,杨阳,荚荣..合成染料与染料脱色过氧化物酶Il-DyP4相互作用的光谱分析[J].安徽大学学报（自然科学版）,2024,48(3):91-101,11.基金项目
国家自然科学基金资助项目(31970100) （31970100）
