北华大学学报（自然科学版）2024，Vol.25Issue(5)：603-608,6.DOI:10.11713/j.issn.1009-4822.2024.05.007
基于TLR2/MyD88信号通路探究五味子乙素对全身炎症反应综合征的影响
Effect of Schisandrae B on SIRS Based on TLR2/MyD88 Signaling Pathway
摘要
Abstract
Objective To investigate the effect of Schisandrin B on zymosan-induced SIRS in mice through TLR2/MyD88 signaling pathway.Method The model was established by intraperitoneal injection of zymosan.The changes of body temperature and white blood cells were recorded.The levels of TNF-α,IL-1β and IL-6 in serum were detected by enzyme-linked immunosorbent assay.ALT,AST,Cr,BUN in serum were detected with the kits;HE staining was used to observe the pathological changes of each tissue.Western Blot was used to detect the expression levels of TLR2,MyD88,NF-κB,HMGB1 in tissues.Results SchB can make temperature in mice increase significantly(P<0.01),blood cell in mice increase significantly(P<0.01).SchB could significantly reduce the levels of TNF-α,IL-1β,IL-6,ALT,AST,Cr and BUN(P<0.001).Western Blot results showed that SchB could significantly reduce the expression levels of TLR2,MyD88,NF-κB and HMGB1 in tissues(P<0.01).Conclusion SchB can alleviate the SIRS induced by zymosan,and the mechanism is mainly to regulate TLR2/MyD88 signaling pathway to alleviate inflammation.关键词
五味子乙素/全身炎症反应综合征/酵母聚糖/TLR2/MyD88信号通路Key words
Schisandrin B/systemic inflammatory response syndrome/zymosan/TLR2/MyD88 signaling pathway分类
医药卫生引用本文复制引用
许亚威,赵紫涵,李贺,王春梅,孙靖辉,张志宏,陈曦,张成义..基于TLR2/MyD88信号通路探究五味子乙素对全身炎症反应综合征的影响[J].北华大学学报（自然科学版）,2024,25(5):603-608,6.基金项目
北华大学研究生创新计划项目(2022066). （2022066）
