北华大学学报（自然科学版）2024，Vol.25Issue(5)：609-614,6.DOI:10.11713/j.issn.1009-4822.2024.05.008
甘草附子汤对风寒湿痹型痛风性关节炎小鼠的治疗作用及机制
Therapeutic Effect and Mechanism of Licorice Aconite Decoction on Gouty Arthritis in Mice with Wind-cold-damp Obstruction
摘要
Abstract
Objective To investigate the effect of Licorice aconite decoction(LA)on gouty arthritis in mice with wind-cold-damp obstruction.Method Forty ICR mice were randomly divided into normal group(CON),model group(MOD),Febuxostat group(FBX,8mg/(kg·d)),Licorice aconite decoction group(LA,6.4g/(kg·d)).At the same time,gouty arthritis in mice model with wind-cold-damp obstruction was established,lasted two weeks.To observe the joint swelling in mice;The serum levels of uric acid(UA),malondialdehyde(MDA),catalase(CAT)and superoxide dismutase(SOD)were detected;Xanthine oxidase(XOD)level in liver was detected;By ELISA kit to detect serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)level.HE staining was used to observe the histopathological changes of ankle joints.Western blot was used to detect the expression of nuclear factor kappa-B(NF-κB),threonine protein kinase B(AKT),TNF-α and IL-1β protein expression levels in the ankle joint of mice.Results The swelling degree of mice in the FBX group and the LA group was significantly reduced in different degrees(P<0.05).Compared with MOD group,the serum UA concentration,MDA,XOD,TNF-α and IL-1β levels in the FBX and LA groups were significantly decreased(P<0.05),and the CAT and SOD levels were significantly increased(P<0.05).HE staining showed that compared with CON group,the infiltration of inflammatory cells in the joints of MOD group was more obvious.Compared with the MOD group,the inflammatory cell infiltration was reduced in FBX group and LA group,and the cell morphology was relatively smooth and complete.Western blot results showed that compared with MOD group,the protein expression levels of NF-κB,AKT,TNF-α and IL-1β in joint tissues were significantly decreased in FBX group and LA group(P<0.05).Conclusion Licorice aconite decoction has protective effect on gouty arthritis mice with wind-cold-damp obstruction.The mechanism may improve the oxidative stress state in the model mice,inhibit the activation of NF-κB/AKT signaling pathway,and down-regulate the expression of downstream inflammatory factors TNF-α and IL-1β,so as to reduce joint inflammation in mice.关键词
甘草附子汤/风寒湿痹型痛风性关节炎/氧化应激/炎症因子Key words
Licorice aconite decoction/gouty arthritis caused by wind-cold-damp obstruction/oxidative stress/inflammatory factor分类
医药卫生引用本文复制引用
刘小瑜,安丽萍,王曼力,王宝辉,王海珊,闫宇宁,林逢源,管芸卉..甘草附子汤对风寒湿痹型痛风性关节炎小鼠的治疗作用及机制[J].北华大学学报（自然科学版）,2024,25(5):609-614,6.基金项目
吉林省科技攻关计划项目(2023C029-1). （2023C029-1）
