北华大学学报（自然科学版）2024，Vol.25Issue(5)：620-625,6.DOI:10.11713/j.issn.1009-4822.2024.05.010
冠心病患者血清VCAM-1、miR-145、Gal-3、SFRP5水平变化
Changes of Serum VCAM-1,miR-145,Gal-3,SFRP5 Levels in Patients with Coronary Heart Disease
摘要
Abstract
Objective To investigate the change and significance of serum levels of vascular cell adhesion molecule-1(VCAM-1),miR-145,galactose agglutinin 3(Gal-3),and secretory curl protein 5(SFRP5)in patients with coronary heart disease.Method 80 patients with coronary heart disease were selected as the coronary heart disease group,and 40 healthy volunteers were collected as the healthy control group.Collect fasting elbow vein blood in the morning,and measure serum VCAM-1,Gal-3 and SFRP5 concentrations using enzyme-linked immunosorbent assay;RT-PCR was used to detect the expression level of miR-145 in serum.According to the number of lesions diagnosed by coronary angiography,they are divided into single vessel lesions,double vessel lesions and multi vessel lesions.Collect demographic characteristics of subjects in two groups and laboratory indicators of patients with coronary heart disease;Follow up for one year and record the occurrence of adverse prognosis(worsening of the condition,re-admission,death).Results The serum levels of VCAM-1 and Gal-3 of patients in coronary heart disease group were significantly higher than those of healthy control group,while levels of miR-145 and SFRP5 were significantly lower than those of healthy control group(all P<0.01).Serum levels of VCAM-1 and Gal-3 of patients in acute myocardial infarction group were significantly higher than those of patients in unstable angina group and stable angina group,while serum levels of miR-145 and SFRP5 were significantly lower than those of patients in unstable angina group and stable angina group(all P<0.05).Serum levels of VCAM-1 and Gal-3 of patients with multiple vessel lesions were significantly higher than those of patients with double vessel disease and single vessel disease,while serum levels of miR-145 and SFRP5 were significantly lower than those of patients with double vessel disease and single vessel disease(all P<0.05).Serum levels of VCAM-1 and Gal-3 of patients with poor prognosis were significantly higher than those of patients with good prognosis,while levels of miR-145 and SFRP5 were significantly lower than those of patients with good prognosis(all P<0.01).Levels of VCAM-1,miR-145,Gal-3 and SFRP5 were independent prognostic factors for patients with coronary heart disease.ROC curve analysis shows that the combined detection of serum levels of VCAM-1,miR-145,Gal-3 and SFRP5 has high predictive value for the prognosis of patients with coronary heart disease(AUC=0.928).Conclusion Patients with coronary heart disease have high expression of VCAM-1 and Gal-3 levels in serum,while low expression of miR-145 and SFRP5 levels,which are closely related to the classification of coronary heart disease and the number of coronary artery lesions,combined detection has high predictive value for prognosis.关键词
冠心病/血管细胞黏附分子-1/miR-145/半乳糖凝集素-3/分泌型卷曲蛋白5/预后Key words
coronary heart disease/vascular cell adhesion molecule-1/miR-145/galactose agglutinin-3/secretory curl protein 5/prognosis分类
医药卫生引用本文复制引用
陈鑫,张阿莲..冠心病患者血清VCAM-1、miR-145、Gal-3、SFRP5水平变化[J].北华大学学报（自然科学版）,2024,25(5):620-625,6.基金项目
上海市科学技术委员会科技支撑项目(12401904800) （12401904800）
上海市卫生健康委员会科研项目(2021CX031). （2021CX031）
