吉林大学学报（医学版）2024，Vol.50Issue(3)：676-681,6.DOI:10.13481/j.1671-587X.20240311
氯己定对粪肠球菌耐药性和致病性的影响及其机制
Effect of chlorhexidine on drug resistance and pathogenicity of Enterococcus faecalis and its mechanism
摘要
Abstract
Objective:To discuss the effect of long-term use of chlorhexidine on the resistance of Enterococcus faecalis(E.faecalis),and to clarify its mechanism.Methods:The standard strain of E.faecalis was repeatedly exposed to chlorhexidine for 10 generations,and the minimum inhibitory concentration(MIC)was recorded at each passage.The bacteria collected from the 10th generation with increased MIC values were designated as the E.faecalis chlorhexidine-resistant strains(E.faecalis-Cs).The growth curves of two strains were drawn;the morphology of two strains were observed by transmission electron microscope;the number of biofilm formation of two strains was detected by crystal violet staining;the bacterial hydrophobicities of two strains were detected by microbial adhesion to hydrocarbons(MATH)method;the expression levels of S-ribosylhomocysteine lyase(LuxS)mRNA in the bacterial biofilms of two strains were detected by real-time fluorescence quantitative PCR(RT-qPCR)method.Results:From the 0th to the 10th generation,the MIC values of E.faecalis were gradually increased.The growth curves of E.faecalis and E.faecalis-Cs showed no significant differences.The transmission electron microscope observation results showed that both E.faecalis and E.faecalis-Cs appeared oval or diplococcal,with intact cell wall structures,smooth edges,and evenly distributed cytoplasm.There were no significant differences in the morphology,size,cell wall thickness,or integrity between two types of bacteria.The crystal violet staining results showed that compared with E.faecalis,the number of biofilm formation of E.faecalis-Cs was significantly increased(P<0.05).The MATH results showed tha the hydrophobicity of E.faecalis-Cs was significantly higher than that of E.faecalis(P<0.05).The RT-qPCR results showed that the expression level of LuxS mRNA in the biofilms of E.faecalis-Cs was significantly higher than that of E.faecalis(P<0.05).Conclusion:E.faecalis develops the resistance after repeated exposure to the chlorhexidine,and the pathogenicity of the resistant strain is enhanced.The high expressin of quorum sensing(QS)system LuxS gene and stronger biofilm forming ability of bacteria may be the potential mechanism for E.faecalis to tolerate the chlorhexidine.关键词
粪肠球菌/氯己定/耐药性/粪肠球菌氯己定耐药菌株/群体感应系统Key words
Enterococcus faecalis/Chlorhexidine/Drug resistance/Enterococcus faecalis resistance stain/Quorum sensing system分类
生物科学引用本文复制引用
徐智博,姜鑫淼,甄雨琦,白玛曲珍,孙梦瑶,孟秀萍..氯己定对粪肠球菌耐药性和致病性的影响及其机制[J].吉林大学学报（医学版）,2024,50(3):676-681,6.基金项目
吉林省财政厅科技项目(JCSZ2021893-23) （JCSZ2021893-23）
