中国耳鼻咽喉头颈外科2024，Vol.31Issue(6)：341-345,5.DOI:10.16066/j.1672-7002.2024.06.001
头颈部黏膜病损中拭子HPV半定量检测的应用研究
Application of HPV semi-quantitative detection in swab of head and neck mucosal lesions
摘要
Abstract
OBJECTIVE To compare the consistency between the semi-quantitative detection of HPV E6/E7 mRNA and the detection of p16 IHC and E6/E7RNA ISH in the tissues,and the feasibility of detecting high-risk HPV in head and neck mucosal lesions by HPV E6/E7 mRNA detection in the swabs was discussed.METHODS A total of 100 cases of head and neck mucosal lesions treated by the Department of Head and Neck Surgery,Beijing Tongren Hospital Affiliated to Capital Medical University from September 2022 to August 2023 were collected.Semi-quantitative detection of HPV E6/E7 mRNA was performed in oropharynx,lesion surface swab and lesion tissue specimen,and p16 immunohistochemical staining(IHC)and E6/E7 mRNA in situ hybridization(ISH)were detected in lesion tissue,and the consistency and difference of different detection results were studied.RESULTS Among the 100 patients,83 met the inclusion criteria and were divided into 21 papilloma cases,10 polyps/chronic inflammation cases,19 laryngeal cancer cases,13 oropharyngeal cancer cases,and 20 hypopharyngeal cancer cases according to pathological diagnosis.The HPV E6/E7 mRNA semi-quantitative results of oropharyngeal swab and lesion surface swab showed moderate or near high consistency with p16 IHC results.The results of HPV E6/E7 mRNA semi-quantitative in diseased tissue were highly consistent with those of p16 IHC(Kappa=0.780).In the diagnostic efficacy analysis,both swabs showed high consistency with HPV E6/E7 mRNA ISH(Kappa=0.690 and 0.708).CONCLUSION In the head and neck mucosal lesions,the HPV semi-quantitative detection results of oropharyngeal and lesion surface swab showed good consistency compared with classical p16 IHC and gold standard HPV E6/E7 mRNA ISH.It is a simple and reliable method for clinical high-risk HPV detection,which is helpful for the screening and individualized precise prevention and control of HPV infection in head and neck mucosal lesions.关键词
头颈部肿瘤/免疫组织化学/原位杂交/人乳头状瘤病毒/HPV E6/E7Key words
Head and Neck Neoplasms/Immunohistochemistry/In Situ Hybridization/human papilloma virus/HPV E6/E7引用本文复制引用
李齐佳,钟琦,王哓艳,何雨蓉,李荣佳,史晓钰,丁硕,郭伟,赵延明,房居高..头颈部黏膜病损中拭子HPV半定量检测的应用研究[J].中国耳鼻咽喉头颈外科,2024,31(6):341-345,5.基金项目
北京市科技新星交叉合作课题(20230484484) （20230484484）
北京市自然科学基金面上项目(7242039) （7242039）
