吉林医药学院学报2024，Vol.45Issue(4)：246-249,4.
紫苏叶提取物对小鼠止咳祛痰作用研究
Study on the Antitussive and Expectorant Effects of Perilla Frutescens Leaves Extracts in Mice
摘要
Abstract
Objective Investigate the efficacy of Perilla frutescens leaves extracts prepared by aqueous extraction and ethanol extraction methods on antitussive and expectorant effects in mice.Methods P.frutescens leaves extracts were prepared using water and 50%etha-nol as solvents.The differences in total phenols,total flavonoids,total triterpenes,and total polysaccharides contents among the various P.frutescens leaves extracts were compared.In addition,the antitussive effects of the P.frutescens leaves extracts were evaluated using the concentrated ammonia-induced cough test and the guaifenesin-induced tracheal phenol red secretion method in mice.The parameters ob-served included the cough latency,cough frequency,and tracheal phenol red secretion.Results The content of total phenols,total flavo-noids,and total triterpenes in the 50%ethanol extract of P.frutescens leaves were significantly higher than that in the water extract(P<0.01).However,the content of total polysaccharides in the water extract of P.frutescens leaves were significantly higher than that in the 50%ethanol extract(P<0.01).Compared to the model group,the water extract of P.frutescens leaves significantly prolonged the cough la-tency in mice and significantly reduced the number of coughs induced by concentrated ammonia(P<0.01).The cough latency of mice treated with the 50%ethanol extract of P.frutescens leaves showed no significant difference(P>0.05),but the extract significantly de-creased the number of coughs(P<0.01).Compared to the control group,the water extract of P.frutescens leaves significantly increased the tracheal phenol red secretion(P<0.01),while the 50%ethanol extract showed no significant difference in phenol red secretion(P>0.05).Conclusion The composition of P.frutescens leaves extracts varies depending on the solvent used.The water extract of P.frutes-cens leaves is characterized by a higher content of polysaccharides,which exhibit excellent antitussive and expectorant effects.Con-versely,the ethanol extract of P.frutescens leaves is mainly composed of phenols,flavonoids,and triterpenes,and possesses notable anti-tussive effects.关键词
紫苏叶/提取物/止咳/祛痰Key words
Perilla frutescens(L.)leaves/extracts/antitussive activity/expectorant activity分类
医药卫生引用本文复制引用
叶超,陈志莹,宋源,王婷婷,杨福豪,吴盈,范红艳..紫苏叶提取物对小鼠止咳祛痰作用研究[J].吉林医药学院学报,2024,45(4):246-249,4.基金项目
吉林省教育厅科学技术研究项目(JJKH20220467KJ) （JJKH20220467KJ）
吉林省大学生创新创业训练计划项目(S202113706045) （S202113706045）
