吉林医药学院学报2024，Vol.45Issue(4)：250-256,7.
miRNA-200b在糖尿病小鼠海马体中的异常表达及靶基因预测
Abnormal Expression of miRNA-200b in Hippocampus of Diabetes Mice and Prediction of Target Genes
摘要
Abstract
Objective To analyze the expression of miRNA-200b in hippocampus of diabetes mice and predict the target gene of miRNA-200b.Methods Type 1 and type 2 diabetes mouse models were induced by streptozotocin or combined with high-sugar and high-fat diet,respectively.After modeling,the mice were killed and the hippocampus tissues were isolated.The expression of miRNA-200b was detected by fluorescence quantitative PCR.miRNA-200b target genes were predicted by miRWalk,miRDB and miRPathDB gene data-bases.Then,GO analysis and KEGG analysis were performed.Results The diabetes mouse model was successfully established,and the expression levels of miRNA-200b in the hippocampus of diabetic mice were decreased compared with that of normal mice(P<0.05).Bio-informatics prediction showed that three target genes of miRNA-200b were screened,namely GAB1,SLC5A3 and GICCL1.The functions of target genes were concentrated in the signaling pathways of glucose metabolism and neuronutrition.Conclusion The results of this study suggest that miRNA-200b is significantly decreased in the hippocampus of diabetic mice,which is expected to be a new target for diabetes treatment.关键词
糖尿病/海马体/miRNA-200b/靶基因预测Key words
diabetes mellitus/hippocampus/miRNA-200b/target gene prediction分类
生物科学引用本文复制引用
马广辉,李汶柯,蒙鑫,王会岩,董媛..miRNA-200b在糖尿病小鼠海马体中的异常表达及靶基因预测[J].吉林医药学院学报,2024,45(4):250-256,7.基金项目
吉林省科技厅项目(YDZJ202301ZYTS117) （YDZJ202301ZYTS117）
