吉林医药学院学报2024，Vol.45Issue(4)：257-260,4.
UFLC法测定黄芪桂枝五物汤与芍药水提物中芍药苷和芍药内酯苷的含量
Determination of Paeoniflorin and Albiflorin in Huangqi Guizhi Wuwu Decoc-tion and Paeoniae Radix Alba Water Extract by UFLC
摘要
Abstract
Objective To establish an ultra-fast liquid chromatography method for the determination of paeoniflorin and albiflorin in Huangqi Guizhi Wuwu Decoction(HGWWD)and Paeoniae Radix Alba water extract.Methods Shim-Pack XR-ODS chromatographic column was used,0.1%phosphoric acid water(A)and acetonitrile(B)were adopted as mobile phase,and gradient elution program(0-5 min,10%-20%B;5-10 min,20%-25%B)was applied for good separation.The equilibrium time was 5 min,the flow rate of the mo-bile phase was 0.4 mL/min,the temperature of the chromatographic column was set to 35℃,the wavelength of the UV detector was set to 230 nm,and the injection volume was set to 5 μL.Results The linear range of paeoniflorin and albiflorin was 0.5-20 mg/L.The devel-oped analytical method has excellent linear relationship,good precision,good stability and stable recovery.The contents of paeoniflorin were 2.75-3.84 g/L and 4.70-4.99 g/L,and the contents of albiflorin were 0.632-0.928 g/L and 0.729-0.870 g/L in HGWWD and Pae-oniae Radix Alba water extract.Conclusion The content of paeoniflorin in HGWWD was less than that in the water extract of Paeoniae Radix Alba,indicating that other medicinal ingredients have an inhibitory effect on the extraction of paeoniflorin.There was no significant difference in the content of albiflorin in HGWWD and in the water extract of Paeoniae Radix Alba.This method has the advantages of short analysis time and good stability,and can be used for quality control and compatibility study of HGWWD.关键词
黄芪桂枝五物汤/芍药/芍药苷/芍药内酯苷/超快速液相色谱法Key words
Huangqi Guizhi Wuwu decoction/Paeoniae Radix Alba/paeoniflorin/albiflorin/ultra-fast liquid chromatography method分类
医药卫生引用本文复制引用
关皎,刘昊,李安民,贾洪权,郭宏莉,叶琦,朱倩,张金宇,朱鹤云..UFLC法测定黄芪桂枝五物汤与芍药水提物中芍药苷和芍药内酯苷的含量[J].吉林医药学院学报,2024,45(4):257-260,4.基金项目
国家自然科学基金项目(81703683) （81703683）
吉林省科技厅项目(YDZJ202101ZYTS074,YDZJ202301ZYTS188) （YDZJ202101ZYTS074,YDZJ202301ZYTS188）
吉林省人社厅创新创业人才项目(2023QN35) （2023QN35）
吉林医药学院大学生创新创业训练计划项目(X202313706075) （X202313706075）
