医学分子生物学杂志2024，Vol.21Issue(4)：293-299,7.DOI:10.3870/j.issn.1672-8009.2024.04.001
GATA3通过调控LIFR抑制人乳腺癌细胞的迁移能力
GATA3 Inhibits Migration of Human Breast Cancer Cells by Regula-ting LIFR
摘要
Abstract
Objective To explore the effect of GATA binding protein 3 on the migration ability of breast cancer cells.Methods Lentivirus-mediated GATA3-knockdown cell line was established to study the expression levels and function of GATA3 by performing real-time quantitative fluorescent PCR,Western blotting and Transwell assay in vitro.The binding sites of GATA3 in LIFR promoter region was detected by Chromatin immunoprecipitation assay(ChIP-qPCR)assay in MCF7 and T47D cells.LIFR was overexpressed in MCF7 cells with reduced GATA3 expression,and the migra-tion capacity of MCF7 cells was measured by Wound healing assay and Transwell assay.Results Compared with the control group,the group of MCF7 cells that knocked down GATA3 had enhanced migration ability(all P<0.05),and decreased expression of LIFR(all P<0.05).ChIP-qPCR data showed a physical binding of GATA3 on the promoter region of LIFR in MCF7 and T47D cells(all P<0.05).Overexpression of LIFR rescued the enhanced cell migration induced by depletion of GATA3 in MCF7 cells(all P<0.05).Conclusion GATA3 inhibits the migration of breast cancer cells MCF7 through transcriptional activation of LIFR.关键词
乳腺癌/GATA结合蛋白3/白血病抑制因子受体LIFR/细胞迁移Key words
breast cancer/GATA binding protein 3/leukemia inhibitory factor receptor/cell migration分类
医药卫生引用本文复制引用
张璐,张瑞,刘俊,杨安钢..GATA3通过调控LIFR抑制人乳腺癌细胞的迁移能力[J].医学分子生物学杂志,2024,21(4):293-299,7.基金项目
国家重点实验室自主研究课题(No.CBSKL2022ZZ04) This work was supported by a grant from the Independent Research Project of State Key Laboratory(No.CBSKL2022ZZ04) （No.CBSKL2022ZZ04）
