医学分子生物学杂志2024，Vol.21Issue(4)：309-314,6.DOI:10.3870/j.issn.1672-8009.2024.04.003
细胞外组蛋白调控TLR4/NF-κB途径对膀胱癌细胞上皮间质转化和侵袭的影响
Effect of Extracellular Histone on Epithelial Mesenchymal Transfor-mation and Invasion of Bladder Cancer Cells by Regulation of TLR4/NF-κB Pathway
摘要
Abstract
Objective To investigate the effect of extracellular histone on epithelial mesenchy-mal transformation(EMT)and invasion of bladder cancer cells by regulating toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)pathway.Methods T24 bladder cancer cells were cultured and divided into 7 groups:control group,histone H3 groups with different concentrations(50,100,200 μg/mL),solvent control(0.1%DMSO)group,histone H3(200 μg/mL)+sol-vent control(0.1%DMSO)group,and histone H3(200 μg/mL)+NF-κB inhibitor Bay11-7082(10 μmol/L)group.The number of invasion cells and the protein expression levels of E-cad-herin,N-cadherin,Vimentin,TLR4 and phosphorylated P65 NF-κB(p-P65 NF-κB)were de-tected 24 h after administration.Results The number of invasion cells and the expression levels of N-cadherin,Vimentin,TLR4 and p-P65 NF-κB in the histone H3 groups were higher than those in the control group,and the protein expression level of E-cadherin was lower than that in the control group(P<0.05),the number of invasion cells and the expression levels of those proteins were changed with the concentration of histone H3 treatment in a dose-dependent manner.The number of cell invasion and the protein expression levels of N-cadherin,Vimentin and p-P65 NF-κB in the histone H3+Bay11-7082 group were lower than those in the histone H3+solvent control group,and the expression level of E-cadherin was higher than that in the histone H3+solvent control group(P<0.05).Conclusion Extracellular histone promotes EMT and invasion of bladder cancer cells,which is related to the activation of TLR4/NF-κB pathway.关键词
膀胱癌/细胞外组蛋白/上皮间质转化/侵袭/TLR4/NF-κB途径Key words
bladder cancer/extracellular histone/epithelial mesenchymal transformation/invasion/TLR4/NF-κB pathway分类
医药卫生引用本文复制引用
韩志军,舒林飞,易湘锰,谭华俊,郑国球,杨帆..细胞外组蛋白调控TLR4/NF-κB途径对膀胱癌细胞上皮间质转化和侵袭的影响[J].医学分子生物学杂志,2024,21(4):309-314,6.基金项目
2023年度湖南省自然科学基金(No.2023JJ50215) This work was supported by 2023 Natural Science Foundation Project of Hunan Province(No.2023JJ50215) （No.2023JJ50215）
