MRPL21调控YAP1/TAZ通路对非小细胞肺癌A549细胞活性的影响OACSTPCD
Effect of MRPL21 on Activity of Non-small Cell Lung Cancer A549 Cells by Regulating YAP1/TAZ Pathway
目的 探究MRPL21 与YAP1/TAZ通路对非小细胞肺癌A549 细胞活性的影响,以期为非小细胞肺癌的治疗提供新思路.方法 非小细胞肺癌组织及癌旁的组织取自2021 年6 月～2023 年9 月于安徽医科大学第一附属医院接受手术治疗的9 例非小细胞肺癌患者,通过免疫组化实验方法分析非小细胞肺癌组织中MRPL21 的表达水平.将非小细胞肺癌A549 细胞分为随机分为 3 组:siNC组、siMRPL21 组以及BPD-MA组.CCK-8 实验与Hoechst染色检测 A549 细胞增殖情况;Transwell实验分析 A549 细胞的侵袭能力;TUNEL染色实验检测A549 细胞的凋亡情况;蛋白质印迹实验检测A549 细胞中MRPL21、YAP1、TAZ蛋白的表达水平.结果 免疫组化实验结果显示,与癌旁的组织比较,非小细胞肺癌组织中的MRPL21 表达上调(P<0.05).与siNC组的A549 细胞比较,CCK-8 实验与Hoechst染色实验结果显示,siMRPL21 组以及BPD-MA组的A549 细胞增殖能力减弱(P<0.05).Transwell实验结果显示,siMRPL21 组以及BPD-MA组的A549 细胞侵袭能力减弱(P<0.05).TUNEL染色实验结果显示,siMRPL21 组以及BPD-MA组的A549细胞凋亡率增加(P<0.05).蛋白质印迹实验结果显示,siMRPL21 组以及 BPD-MA 组的 A549 细胞的YAP1、TAZ蛋白水平表达均明显降低(P<0.05).结论 MRPL21 在非小细胞肺癌组织中高表达;抑制MRPL21 的表达后,A549 细胞的增殖活性、侵袭能力减弱,细胞凋亡率增加,并且这一结果与调控YAP1/TAZ信号通路相关,MRPL21 有望成为治疗非小细胞肺癌的新靶点.
Objective To investigate the effect of MRPL21 and YAP1/TAZ pathway on the activity of non-small cell lung cancer A549 cells,so as to provide new ideas for the treatment of non-small cell lung cancer.Methods Non-small cell lung cancer tissues and adjacent tissues were obtained from 9 patients with non-small cell lung cancer who underwent surgical treatment in the First Affiliated Hospital of Anhui Medical University between June,2021 and September,2023.The expression of MRPL21 in the tissues was analyzed by immunohistochemistry.A549 cells were ran-domly divided into three groups:siNC group,siMRPL21 group and BPD-MA group.CCK-8 assay and Hoechst staining were used to detect the proliferation of A549 cells.Transwell assay was used to analyze the invasion ability of A549 cells.TUNEL staining was used to detect the apoptosis of A549 cells.Western blotting assay was used to detect the expression levels of MRPL21,YAP1 and TAZ proteins in A549 cells.Results The results of immunohistochemistry showed that the expression of MRPL21 was up-regulated in the non-small cell lung cancer tissues compared with that in the adja-cent tissues(P<0.05).Compared with the A549 cells in the siNC group,cells in the siMRPL21 group and the BPD-MA group showed decreased proliferation ability as detected by CCK8 assay and Hoechst staining assay(P<0.05).Transwell assay showed that the invasion abilities of A549 cells in the siMRPL21 group and the BPD-MA group were decreased(P<0.05).TUNEL staining showed that the apoptosis rates of A549 cells were increased in the siMRPL21 group and the BPD-MA group(P<0.05).Western blotting results showed that the siMRPL21 group and the BPD-MA group had significant reduced protein levels of YAP1 and TAZ in A549 cells(P<0.05).Conclu-sion MRPL21 is highly expressed in non-small cell lung cancer tissues.After inhibiting the expres-sion of MRPL21,the proliferation activity and invasion ability of A549 cells are weakened,and the apoptosis rate is increased,which is related to the regulation of YAP1/TAZ signaling pathway.MRPL21 is expected to be a new target for the treatment of non-small cell lung cancer.
方汉林;潘华光;陈宇;张仁泉
安徽医科大学第一附属医院普胸外科 合肥市,230011
临床医学
MRPL21YAP1TAZ非小细胞肺癌A549细胞增殖凋亡侵袭
MRPL21YAP1TAZnon-small cell lung cancerA549 cellsproliferationapoptosisinvasion
《医学分子生物学杂志》 2024 (004)
329-333 / 5
安徽省教育厅高校科学研究项目(No.KJ2019ZD22) This work was supported by a grant from the University Scientific Research Project of Anhui Provincial Department of Education(No.KJ2019ZD22)
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