医学分子生物学杂志2024，Vol.21Issue(4)：341-346,6.DOI:10.3870/j.issn.1672-8009.2024.04.008
神经生长因子联合BMSCs-源外泌体通过Keap1/NQO1/Nrf2信号通路缓解脑出血大鼠神经损伤
Nerve Growth Factor Combined with BMSCs Exosomes Alleviates Neural Injury in Rats with Intracerebral Haemorrhage through Keap1/NQO1/Nrf2 Signaling Pathway
摘要
Abstract
Objective To explore the therapeutic effect and mechanisms of rat nerve growth factor(RtNGF)combined with bone marrow mesenchymal stem cell-derived exosomes(BMSCs exosomes)on neural injury of intracerebral haemorrhage(ICH)rats.Methods The BMSCs exo-somes were prepared for the following experiments.Sixty rats were randomly divided into 5 groups:Sham group,ICH group,ICH+RtNGF group,ICH+BMSCs exosomes group,and ICH+RtNGF+BMSCs exosomes group,with 12 rats in each group.A ICH rat model was established and RtNGF or BMSCs exosomes were treated alone or in combination.Paraffin embedded tissue sections of rat brain tissues in each group were prepared and HE staining was performed.qPCR and Immunohistochemis-try were used to determine the mRNA and protein expression levels of genes in the Keap1/NQO1/Nrf2 signaling pathway.Results Significant existence of tissue lacunes and blood clots were ob-served in the ICH group rather than in the Sham group.RtNGF or/and BMSCs treatments improved that situation,and the combination of RtNGF and BMSCs exosomes treatment had the best effect on alleviating neural injury.The mRNA expression levels of Keap1,NQO1 and Nrf2 in the brain tis-sues,and the IHC relative staining scores of the three proteins in the ICH group were increased when compared with those in the Sham group(all P<0.05).The levels of the above indicators in the ICH+RtNGF group,ICH+BMSCs exosomes group,and ICH+RtNGF+BMSCs exosomes group were decreased when compared with those in the ICH group(P<0.05),with the values the lowest in the ICH+RtNGF+BMSCs exosomes group(P<0.05).Conclusion RtNGF combined with BMSCs exosomes has significant effect on reducing oxidative stress and alleviating neural injury through Keap1/NQO1/Nrf2 signaling pathway in ICH rats.关键词
脑出血/大鼠神经生长因子/BMSCs-源外泌体/Keap1/NQO1/Nrf2信号通路/神经损伤Key words
intracerebral haemorrhage/nerve growth factor/BMSCS exosomes/Keap1/NQO1/Nrf2 signaling pathway/neural injury分类
医药卫生引用本文复制引用
李方,唐仕军,麦麦提依明·托合提..神经生长因子联合BMSCs-源外泌体通过Keap1/NQO1/Nrf2信号通路缓解脑出血大鼠神经损伤[J].医学分子生物学杂志,2024,21(4):341-346,6.基金项目
新疆维吾尔自治区人民医院院内项目(No.20210108) This work was supported by a grant from Xinjiang Uygur Autonomous Region People's Hospital Internal Project(No.20210108) （No.20210108）
