医学分子生物学杂志2024，Vol.21Issue(4)：347-353,360,8.DOI:10.3870/j.issn.1672-8009.2024.04.009
干扰hsa_circRNA_002178基因抑制卵巢癌细胞的恶性生物学行为
hsa_circRNA_002178 Gene Interference Inhibits Malignant Biologi-cal Behavior of Ovarian Cancer Cells
摘要
Abstract
Objective To study the effect of circRNA_002178 gene interference on malignant biological behavior of ovarian cancer cells.Methods Human ovarian cancer SKOV3 cells were di-vided into 4 groups:control group,short hairpin RNA negative control(shRNA-NC)group,cir-cRNA_002178-shRNA 1 group and circRNA_002178-shRNA 2 group.The expression level of cir-cRNA_002178 was detected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR).Flow cytometry was used to detect the cell apoptosis and mitochondrial membrane potential and to select the stem cell positive(CD44,CD133)cell lines.The stem cell spheroidization test was used to detect the sphere formation rate and the diameter of spheres.Transwell assay was used to detect the cell invasion.Western blotting was used to detect the expression levels of the proteins.The transplanted tumor model was constructed to observe the effect of circRNA_002178 gene interference on tumor formation.Results CircRNA_002178 interference significantly promoted the apoptosis of SKOV3 cells,decreased the number of CD44 and CD133 positive cells,the rate of sphere forma-tion,the diameter of spheres and the number of invasive cells,intensified the changes of mitochon-drial membrane potential,and inhibited tumor growth and Akt/mTOR pathway activation in trans-planted tumor rats.Conclusion CircRNA_002178 interference can inhibit the malignant biological behavior of ovarian cancer cells.The mechanism may be related to the inhibitory effect on Akt/mTOR pathway.关键词
circRNA_002178/CD133/线粒体膜电位/细胞凋亡/卵巢癌Key words
circRNA_002178/CD133/mitochondrial membrane potential/apoptosis/o-varian cancer分类
医药卫生引用本文复制引用
彭胡,龙成兰,罗婷,尹帮朝,周剑..干扰hsa_circRNA_002178基因抑制卵巢癌细胞的恶性生物学行为[J].医学分子生物学杂志,2024,21(4):347-353,360,8.基金项目
泸州市科技计划项目(No.2021-S-46) This work was supported by a grant from the Luzhou Science and Technology Plan Project(No.2021-S-46) （No.2021-S-46）
