大麻二酚联合米诺环素对牙周炎治疗作用的实验研究OA北大核心CSTPCD

Objective The therapeutic effect of cannabidiol combined with minocycline on experimental periodontitis in mice was studied in vitro and in vivo.Methods The mouse periodontitis model was established by thread ligation.The oral area was treated with cannabidiol,minocycline,and both.Quantitative reverse transcription polymerase chain re-action(qRT-PCR)was used to detect the changes in inflammatory factors in peripheral blood of mice with periodontitis.Micro CT,western blot(WB),and hematoxylin-eosin(HE)staining were used to analyze the improvement in local in-flammation.Porphyromonas gingivalis(P.gingivalis)bacteriostatic assay was used to detect the antibacterial properties of the combined drugs in vitro.Inflammation of macrophages and gingival fibroblasts was induced in vitro,and mRNA expression related to inflammation was detected by qRT-PCR.The effect of drugs on cell migration was explored by wound-healing assay,and the effect of drugs on colla-gen production was explored by enzyme-linked immu-nosorbent assay.Results After combined administra-tion of cannabidiol and minocycline,the systemic in-flammation index of mice decreased,the local attachment loss was alleviated,the tissue inflammation level was reduced,and the destruction of periodontal soft and hard tissues improved compared with single administration.The results of the antibacterial experiment in vitro showed that the combined drugs had good antibacterial properties.In vitro cell experi-ments proved that the combination of drugs reduced the expression levels of various inflammatory factors in macro-phages,promoted the proliferation and migration of human gingival fibroblasts in inflammatory states,and improved the collagen formation function.Conclusion Compared with the single medication method,the local application of cannabi-diol combined with minocycline has a significant effect on experimental periodontitis in mice.