广西林业科学2024，Vol.53Issue(3)：315-323,9.DOI:10.19692/j.issn.1006-1126.20240306
低共熔溶剂法提取连翘总黄酮工艺研究
Study on Extraction Process of Total Flavonoids from Forsythia suspensa by Deep Eutectic Solvents Method
摘要
Abstract
In order to explore optimum extraction process of total flavonoids from Forsythia suspensa,total flavo-noids from different parts of F.suspensa(leaves,fruits and stems)were extracted by deep eutectic solvents(DESs)method.Different combinations of DESs were configured with choline chloride as hydrogen-bond acceptor and urea,ethylene glycol,glycerol,citric acid,glucose,1,2-propanediol and sorbitol as hydrogen-bond donors re-spectively,and extraction rates of total flavonoids by different combinations were determined.Response surface method was employed to confirm optimum extraction process by adjusting molar ratio of DESs,water content of DESs,liquid-solid ratio,extraction time and extraction temperature as single factor variables,and extraction rate of total flavonoids as evaluation index.Results showed that combination of choline chloride/glycerol had the highest extraction rate of total flavonoids.Content of total flavonoids in F.suspensa fruits was the highest,followed by F.suspensa leaves.Content of total flavonoids in F.suspensa stems was the lowest.The optimum ex-traction process of total flavonoids optimized by response surface method was molar ratio of choline chloride/glycerol 1∶2,water content of DESs 21%,liquid-solid ratio 35 mL/g,extraction time 125 min and extraction temperature 70℃.It was feasible to extract total flavonoids from different parts of F.suspensa by DESs method,which could provide theoretical and experimental supports for extraction and deep development of total flavo-noids from F.suspensa.关键词
总黄酮/低共熔溶剂/响应面法/提取工艺/连翘Key words
total flavonoids/deep eutectic solvent/response surface method/extraction process/Forsythia suspensa分类
农业科学引用本文复制引用
周伟,梁旭华,杨文婷,程敏..低共熔溶剂法提取连翘总黄酮工艺研究[J].广西林业科学,2024,53(3):315-323,9.基金项目
陕西省科技创新团队项目(2022TD-56) （2022TD-56）
陕西省重点研发计划项目(2024NC-YBXM-263) （2024NC-YBXM-263）
陕西省教育厅服务地方专项科研计划项目(22JC031) （22JC031）
陕西省中医药管理局秦创原中医药创新研发转化项目(2022-QCYZH-042) （2022-QCYZH-042）
