国际老年医学杂志2024，Vol.45Issue(4)：397-402,6.DOI:10.3969/j.issn.1674-7593.2024.04.003
西洋参红景天复配方对氧化损伤模型小鼠的抗氧化作用及机制
Antioxidant Effect and Mechanism of American Ginseng Rhodiola Formula on Oxidative Damage Model Mice
摘要
Abstract
Objective To evaluate the antioxidant effect of American ginseng Rhodiola formula on ethanol-induced oxidative damage in model mice,and to explore its mechanism of action,providing a scientific basis for the development of healthy food.Methods Mice were randomly divided into blank control group,acute oxidative injury model group,low dose(330 mg/kg),medium dose(660 mg/kg)and high dose(990 mg/kg)groups according to body weight,10 mice in each group.Each dose group was given test samples by oral gavage.Blank control group and model group were given equal volume of distilled water.After continuous gavage for 30 days,50%ethanol was intragastrically administered at12 mL/kg after fasting for 16 h in each group except blank control group.Blood and liver were collected from mice 6 h later.Malondialdehyde(MDA),8-epoxyisoprostane(8-Isoprostane),protein carbonyl(PCO),glutathione(GSH),aspartate aminotransferase(AST),alanine aminotransferase(ALT),triglyceride(TG),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)content and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity in mouse serum,MDA,PCO,GSH content and SOD activity in liver tissue were measured,and the protein expression of p-p38,p-ERK and p-JNK was detected by Western blotting..Results Compared with the model group,the MDA content in the ser-um of mice in the low-dose group of American ginseng Rhodiola formula decreased(P<0.05),the 8-Isoprostane and PCO contents in each dose group decreased(P<0.05),the GSH content and GSH-Px and SOD activities in each dose group increased(P<0.05),the TNF-α,IL-1β,and IL-6 levels in each dose group decreased(P<0.05),and the ALT and AST activities in each dose group de-creased(P<0.05).The MDA content in the liver of mice in the low and medium dose groups decreased(P<0.05),the PCO and TG contents in each dose group decreased(P<0.05),and the SOD activity in the low and medium dose groups and the GSH content in the low dose group increased(P<0.05).The low dose group could inhibit the expression of p-p38 and p-JNK(P<0.05),and the medium and high dose groups could inhibit the expression of p-p38,p-ERK and p-JNK(P<0.05).Conclusion Rhodiola rosea,American ginseng,acerola cherry powder and grape seed extract composition of medicinal food homologous complex formula has a strong antioxidant health care effect.关键词
抗氧化/西洋参红景天复配方/酒精性肝损伤Key words
Antioxidant/American ginseng Rhodiola formula/Alcoholic liver injury引用本文复制引用
冯颖童,扈觐玺,刘佳琪,刘双巧,贾岚,冯广旭,王景霞..西洋参红景天复配方对氧化损伤模型小鼠的抗氧化作用及机制[J].国际老年医学杂志,2024,45(4):397-402,6.基金项目
国家重点研发计划中医药现代化研究重点专项(2018YFC1706803) （2018YFC1706803）
