国际老年医学杂志2024，Vol.45Issue(4)：419-426,8.DOI:10.3969/j.issn.1674-7593.2024.04.007
神经生长因子β在糖尿病周围神经病变中的作用
Effect of Nerve Growth Factor β on Diabetic Peripheral Neuropathy
摘要
Abstract
Objective To investigate the mechanism of nerve growth factor β(NGF β)in diabetic peripheral neuropathy(DPN).Methods High glucose cell model was established and divided into control group,OE-NGFβ group and NC-NGFβ group.Diabetic neuropathy model was established and divided into rat control group,OE-NGFβ group and NC-NGFβ group.RT-qPCR and Western blot were used to detect the expression changes of insulin-like growth factor-1(IGF-1),intercellular adhesion molecule-1(ICAM-1),tumor necrosis factor-α(TNF-α),autophagy-related gene 12(ATG12),microtubule-associated protein 1A/1B light chain 3B(LC3B)and P62 in cells and rat sciatic nerves.The levels of malondialdehyde(MDA),nitric oxide(NO),reactive oxygen species(ROS)and superoxide dismutase(SOD)in blood samples of DPN rats were detected by ELISA.Results Compared with control group,the mRNA and protein levels of IGF-1,ATG12,LC3B and P62 in the OE-NGFβ group were significantly increased(P<0.001),while the mRNA and protein levels of ICAM-1 and TNF-α were significantly decreased(P<0.001).Compared with control group,the mRNA and protein levels of IGF-1,ATG12,LC3B and P62 in the sciatic nerve of the rat OE-NGFβ group were sig-nificantly increased(P<0.001),and the mRNA and protein levels of ICAM-1 and TNF-α were significantly decreased(P<0.001).In addition,compared with control group,the levels of MDA,NO,and ROS were significantly decreased(P<0.001)and the levels of SOD were significantly increased(P<0.001)in the rat OE-NGFβ group.Conclusion NGFβ may exert neuroprotective effects in DPN by regulating autophagy and antioxidant,suggesting that NGFβ may be a potential therapeutic target for DPN.关键词
糖尿病神经病变/神经生长因子β/自噬/抗氧化Key words
Diabetes peripheral neuropathy/Nerve growth factor β/Autophagy/Antioxidant引用本文复制引用
陆春晖,张敬敬,陶慧文,罗梅,罗荔..神经生长因子β在糖尿病周围神经病变中的作用[J].国际老年医学杂志,2024,45(4):419-426,8.基金项目
新疆维吾尔自治区自然科学基金(2022D01C317) （2022D01C317）
