南方农业学报2024，Vol.55Issue(4)：964-972,9.DOI:10.3969/j.issn.2095-1191.2024.04.006
辣椒根际土壤细菌群落与理化性质互作分析
Interaction analysis of bacterial communities and physicochemi-cal properties in rhizosphere soil of pepper
摘要
Abstract
[Objective]To analyze interaction between pepper rhizosphere soil bacterial community structure and physicochemical properties,which could provide reference for scientific prevention and control of soil-borne diseases and high yield and high quality cultivation of pepper.[Method]In this study,the rhizosphere soils of pepper plants at seedling stage(SD),flowering stage(FR),and fruiting stage(FT)were investigated.The pH,organic matter,total nitrogen,al-kaline nitrogen,total phosphorus,available phosphorus,total potassium,and available potassium contents in three soil samples were determined.High-throughput sequencing using the Illumina HiSeq 2500 platform was performed to analyze the bacterial Alpha diversity indexes,relative abundance at phylum and genus levels in the rhizosphere soils of pepper plants at different growth stages.Principal coordinate analysis(PCoA)and dissimilarity analysis based on Bray-Curtis dis-tance was conducted to assess the bacterial communities among different growth stages and the correlation with physico-chemical factors.[Result]The results showed significant differences in pH and alkaline nitrogen content among SD,FR and FT as pepper plants grew(P<0.05,the same below).The Alpha diversity indexes,richness and Chao1 index of soil bacterial communities in the three growth stages showed no significant differences(P>0.05).The SD soil had the highest number of unique operational taxonomic units(OTUs).Proteobacteria,Actinobacteria,Acidobacteria,Bacteroidetes,and Cyanobacteria were dominant phyla in the rhizosphere soils of the three growth stages.Streptophyta,Acidovorax,Ni-trososphaera and Gp6 had relatively high abundance in the SD rhizosphere soil.Streptophyta,Nitrososphaera and Rhodo-planes had relatively high abundance in the FR rhizosphere soil.Streptophyta and Trinickia were dominant genera in the FT rhizosphere soil.Principal coordinate analysis(PCoA)and dissimilarity analysis revealed significant differences among bacterial communities in rhizosphere soil samples from different growth stages.The correlation analysis showed ex-tremely significant and strong positive correlation between pH and alkaline nitrogen with bacterial communities(P<0.01).[Conclusion]The physical and chemical properties of pepper rhizosphere soil and the structure of bacterial community vary with different growth stages.PH and alkaline nitrogen are key factors influencing the change in bacterial communi-ties.关键词
生育期/根际/细菌群落/高通量测序Key words
growth stage/rhizosphere/bacterial community/high-throughput sequencing分类
农业科技引用本文复制引用
罗路云,王殿东,赵志祥,廖静静,江波,张卓..辣椒根际土壤细菌群落与理化性质互作分析[J].南方农业学报,2024,55(4):964-972,9.基金项目
国家自然科学基金项目(32272517) （32272517）
重庆市自然科学基金项目(CSTB2022NSCQ-MSX0307) （CSTB2022NSCQ-MSX0307）
湖南省农业科技创新资金项目(2022CX68) （2022CX68）
重庆市教育委员会项目(KJQN202001433) National Natural Science Foundation of China(32272517) （KJQN202001433）
Chongqing Natural Science Founda-tion(CSTB2022NSCQ-MSX0307) （CSTB2022NSCQ-MSX0307）
Hunan Agricultural Science and Technology Innovation Fund Project(2022CX68) （2022CX68）
Scientific and Technological Research Project of Chongqing Education Commission(KJQN202001433) （KJQN202001433）
