南方农业学报2024，Vol.55Issue(4)：985-995,11.DOI:10.3969/j.issn.2095-1191.2024.04.008
硒砂瓜根系不同生态位细菌群落多样性及结构分析
Bacterial community diversity and structure in different ecological niches of selenium watermelon root
摘要
Abstract
[Objective]Analyzing the bacterial community structure and diversity,in order to understand the micro-bial composition rule and explore microbial resources in different ecological niches of selenium watermelon root-associated microbiome as well as provide theoretical reference for maintaining soil health and microbial ecosystem stabi-lity of selenium watermelon.[Method]Five point sampling method was used to collect soil samples from four ecological niches,including root zone,rhizosphere,rhizoplane and endosphere,labeled as SRW,SRJ,SRB and SRN.The Illumina Miseq sequencing platform was used to analyze the bacterial community in different niches of the selenium watermelon root.The Alpha diversity index of bacterial community were explored,including Sobs,Shannon,Simpson,Chao1,and ACE indexes.Futher,the differences at the phylum and genus levels between each ecological niche,endophytic bacteria and other niches in the selenium watermelon root were compared.Principal coordinate analysis(PCoA)and traceability analysis were performed.[Result]The results showed that the richness and diversity of bacterial communities in soil(SRW,SRJ and SRB)were significantly higher than those in endophytic bacterial communities(SRN)(P<0.05,the same below).Diversity was ranked by Shannon and Simpson indexes as SRJ>SRW>SRB>SRN,while richness was ranked by ACE and Chao1 indexes as SRW>SRJ>SRB>SRN.The dominant bacterial phyla in the roots of selenium water-melon were Proteobacteria and Actinobacteriota.The proportion of Proteobacteria in all niches ranged from 59.59% to 84.62% .The dominant genera in four niches were Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,Klebsiella and Azotobacter.The proportion of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium was 5.48%-15.68% .PCoA analy-sis showed that there were significant differences in the community structure of selenium watermelon root in diffeferent niches(R2=0.6651,P=0.001).There were significant differences in the abundance of 10 bacterial phyla and 8 bacterial genera among the top 15 species in relative abundance of root ecological niches.Among the top 15 species in relative abundance of endophytic bacteria and other ecological niches in the selenium watermelon root,there were significant dif-ferences in the abundance of 8 bacterial phyla and 5 bacterial genera.The results of traceability analysis showed that 55.70% of the rhizosphere bacteria came from the root zone,65.75% of the rhizoplane bacteria community came from the rhizosphere bacteria and 90.75% of the bacteria community inside root came from the rhizoplane bacteria,indicating that there was a certain correlation between the sources of bacterial communities in selenium watermelon root system in different niches.[Conclusion]Different bacterial communities of Ningxia selenium watermelon are rich in composition,relative abundances are different and bacterial community sourcesb are related,and different niches can select and filter bacterial communities.关键词
硒砂瓜/根系细菌群落/生态位/多样性/群落结构Key words
selenium watermelon/root bacterial community/niche/diversity/community structure分类
农业科技引用本文复制引用
朱滕滕,岳思君,谢志鹏,王国琴,郑蕊,苏建宇..硒砂瓜根系不同生态位细菌群落多样性及结构分析[J].南方农业学报,2024,55(4):985-995,11.基金项目
国家自然科学基金项目(32160023) （32160023）
宁夏重点研发计划项目(2022BBF02010) （2022BBF02010）
中央引导地方科技发展资金项目(2023FRD05032) （2023FRD05032）
宁夏大学生创新创业训练计划项目(S202310749087) National Natural Science Foundation of China(32160023) （S202310749087）
Ningxia Key Research and Develop-ment Plan Project(2022BBF02010) （2022BBF02010）
Central Guidance for Local Scientific and Technological Development Funding Pro-ject(2023FRD05032) （2023FRD05032）
Ningxia College Student Innovation and Entrepreneurship Training Project(S202310749087) （S202310749087）
