南方农业学报2024，Vol.55Issue(4)：1023-1034,12.DOI:10.3969/j.issn.2095-1191.2024.04.011
火龙果茎溃疡病植株茎组织细菌群落特征分析
Characteristics of bacterial community in the stems of canker diseased pitaya plants
摘要
Abstract
[Objective]The aim was to compare the characteristics and functional differences of the endophytic bacte-rial communities in the stem tissues of healthy and canker diseased pitaya plants,so as to provide theoretical basis for re-vealing the occurrence mechanism of pitaya canker and studying the prevention and comprehensive control techniques.[Method]Stem tissues of healthy and canker diseased pitaya plants were collected,and the 16S rDNA sequences of endo-phytic bacteria in the stem tissues were determined using Illumina Miseq sequencing platform to analyze the differences of bacterial community characteristics in the stems of healthy and diseased plants.[Result]The bacterial community species and amplicon sequence variants(ASVs)in the stems of healthy pitaya plants were lower than those of diseased plants,there was no significant difference in bacterial community diversity between healthy plants and diseased plants(P>0.05).Among the bacterial dominant bacterial phyla,the relative abundances of Actinobacteriota,Chloroflexi and Deinococcota in the stem tissue of diseased plants were higher,while the relative abundance of Bacteroidota in the stem tissue of healthy plants was higher.There were 27 dominant bacterial genera in the stem tissue of diseased plants,and the relative abundance of Pseudomonas,Bacillus,Sphingomonas,Deinococcus,Clostridium_sensu_stricto_1,Curtobacterium,Xanthomonas,Lachnoclostridium,Microvirga,Lysobacter,Klenkia and Paenibacillus was higher.There were 26 domi-nant bacterial genera in the stem tissue of healthy plants,and the relative abundance of norank_f_Muribaculaceae,Bacte-roides,Lactobacillus,Methylobacterium-Methylorubrum,unclassified_f_Xanthomonadaceae,Parabacteroides,Ralsto-nia,Rhodococcus,Streptomyces,Bifidobacterium,Acinetobacter and Cellvibrio was higher.The results of linear dis-criminant analysis(LEfSe)showed that there were more indicator bacteria in the stem tissue of healthy plants than those in the stem tissue of diseased plants,while the indicator bacteria in the stems of diseased plants only belonged to four dif-ferent genera,namely Xanthomonas,Curtobacterium,Lachnochlorostridium and Novosphingobium.The results of bacte-rial function prediction showed that the gene copy numbers of endophytic bacteria in the four secondary functional layers of membrane transport,carbohydrate metabolism,other amino acid metabolism and other secondary metabolite biosyn-thesis were significantly higher than those in healthy plant stems(P<0.05).[Conclusion]After the occurrence of pitaya stem canker,there is no obvious change in the diversity of the endophytic bacterial community in the stem tissues.How-ever,the proportions of some dominant bacterial phyla and genera change obviously.Among them,four indicator bacte-rial genera may be related to the occurrence of stem ulcer disease.关键词
火龙果/溃疡病/茎组织/细菌群落/多样性Key words
pitaya/canker/stem tissue/bacterial community/diversity分类
农业科技引用本文复制引用
梁洲勇,姚姿婷,曹雪颖,韦增亭,赵渊,朱桂宁,邹承武..火龙果茎溃疡病植株茎组织细菌群落特征分析[J].南方农业学报,2024,55(4):1023-1034,12.基金项目
广西自然科学基金项目(2023GXNSFAA026271) （2023GXNSFAA026271）
广西农业科学院基本科研业务专项(桂农科2023YM05) （桂农科2023YM05）
广西农业科学院科技发展基金项目(桂农科2021ZX28) Guangxi Natural Science Foundation(2023GXNSFAA026271) （桂农科2021ZX28）
Basic Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2023YM05) （Guinongke 2023YM05）
Science and Technology Development Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021ZX28) （Guinongke 2021ZX28）
