南方农业学报2024，Vol.55Issue(4)：1060-1069,10.DOI:10.3969/j.issn.2095-1191.2024.04.014
基于iTRAQ蛋白质组学的OsAAP6不同转基因水稻胚乳差异蛋白筛选
Screening differential proteins in OsAAP6 different transgenic rice endosperms based on iTRAQ proteomics
摘要
Abstract
[Objective]The purpose of the study was to screen the differential proteins in the endosperm of different OsAAP6 transgenic rice based on iTRAQ proteomics,and to clarify the differential proteins in the endosperm,in a bid to provide a theoretical basis for the future use of the OsAAP6 gene to enhance the nutritional quality of rice.[Method]Using rice OsAAP6 overexpression positive[OX(+)]and negative control[OX(-)]materials,as well as OsAAP6 complemen-tary expression positive[ZpZc(+)]and negative control[ZpZc(-)]experimental materials,the isotope marker relative and absolute quantification(iTRAQ)technology was used for proteomic detection and analysis.And the contents of four types of storage proteins(gluten,gliadin,albumin and globulin)in its endosperm was detected using the Coomassie bril-liant blue G-250 method.[Result]Compared with the ZpZc(-)and OX(-)transgenic negative control,the expression of OsAAP6 gene in ZpZc(+)and OX(+)transgenic positive endosperm was extremely significantly increased at P<0.01 and P<0.001 levels,respectively.Compared with the ZpZc(-)and OX(-)transgenic negative control,a total of 58 differen-tial proteins were simultaneously up-regulated or down-regulated for expression in ZpZc(+)and OX(+)transgenic posi-tive rice,of which 39 proteins significantly increased(P<0.05,the same below),and 19 proteins significantly decreased.Among the 39 co-up-regulated proteins,27 proteins were involved in the synthesis and accumulation of rice seed storage substrates,and 16 up-regulated proteins were involved in the metabolism and accumulation of seed storage proteins.Compared with the ZpZc(-)and OX(-)transgenic negative control,the content of glutelin,gliadin,albumin and globu-lin in the endosperm of ZpZc(+)and OX(+)transgenic positive rice was significantly increased.[Conclusion]Up-regulation of the OsAAP6 gene expression can inhibit the accumulation of proteins related to amylopectin synthesis,but promote the accumulation of storage proteins in the endosperm,thereby increasing the content of gluten,gliadin,albu-min and globulin in the endosperm.It can be used for molecular breeding and genetic improvement of new high protein rice varieties.关键词
水稻/iTRAQ/蛋白质组学/OsAAP6基因/差异蛋白/营养品质Key words
rice/iTRAQ/proteomics/OsAAP6 gene/differential proteins/nutritional quality分类
农业科技引用本文复制引用
彭波,汪全秀,孙晓宇,邱静,彭娟,赵强,孙艳芳,宋晓华,张志国,周伟..基于iTRAQ蛋白质组学的OsAAP6不同转基因水稻胚乳差异蛋白筛选[J].南方农业学报,2024,55(4):1060-1069,10.基金项目
国家自然科学基金项目(U2004141) （U2004141）
河南省科技攻关项目(222102110141,222102110115,212102110249) National Natural Science Foundation of China(U2004141) （222102110141,222102110115,212102110249）
Henan Science and Technology Re-search Project(222102110141,222102110115,212102110249) （222102110141,222102110115,212102110249）
