南方农业学报2024，Vol.55Issue(4)：1070-1078,9.DOI:10.3969/j.issn.2095-1191.2024.04.015
抗稻瘟病水稻恢复系的分子标记辅助选育及抗性鉴定
Molecular marker-assisted selection and resistance evaluation of rice restorer lines resistant to rice blast
摘要
Abstract
[Objective]The purpose of the study was to carry out molecular marker-assisted selection and resistance evaluation of rice restorer lines resistant to rice blast,and to provide excellent genetic resources and technical reference for breeding new hybrid rice varieties with durable,broad-spectrum disease resistance and good quality.[Method]The du-rable and broad-spectrum rice blast resistance genes Pi1,Pi2 and Pigm were introduced into restorer line GuiR1703 by molecular marker-assisted selection and backcross breeding techniques.Single-gene,double-gene and triple-gene intro-gression lines were obtained and subjected to rice blast identification and agronomic traits investigation to analyze the re-sistance effects among different resistance genes to rice blast and the effects of resistance gene introduction on agronomic traits of the recipient parents.[Result]Molecular markers related to Pi1,Pi2 and Pigm genes were detected in the hybrid progenies and backcross populations.The results showed that rice blast resistance genes Pi1,Pi2 and Pigm had been suc-cessfully introduced into GuiR1703.Seven introgression lines carrying rice blast resistance genes with excellent resistance and agronomic traits were screened,showing strong resistance to rice blast.Among the single-gene introgression lines,the resistance effects of rice blast resistance genes were Pigm>Pi2>Pi1.The resistance of double-gene and three-gene in-trogression lines to rice blast were stronger than that of single-gene introgression lines,and the resistance of three-gene in-trogression lines was the strongest.The resistance effects of different gene combinations were Pi1+Pi2+Pigm>Pi2+Pigm>Pi1+Pigm>Pi1+Pi2.There was no significant difference in panicle length and 1000-grain weight between the seven rice blast resistance gene introgression lines and GuiR1703(P>0.05,the same below).The plant height of two rice blast resis-tance gene introgression lines was significantly higher than that of GuiR1703(P<0.05,the same below).The effective panicle per plant of one rice blast resistance gene introgression line was significantly higher than that of GuiR1703.The to-tal grain number per panicle of three rice blast resistance gene introgression lines was significantly higher than that of GuiR1703.The seed setting rate of five rice blast resistance gene introgression lines was significantly lower than that of GuiR1703.The yield per plant of two rice blast resistance gene introgression lines was significantly lower than that of GuiR1703.The yield per plant of four rice blast resistance gene introgression lines was significantly higher than GuiR1703.[Conclusion]Molecular marker-assisted selection can effectively aggregate multiple genes(genes Pi1,Pi2 and Pigm),so that the resistance of the restorer line GuiR1703 to rice blast can be greatly improved.Moreover,A series of genetic materials that are resistant to rice blast and have similar traits to their parental traits are obtained,which can be used as a parent material for breeding hybrid rice with disease resistance and good quality.关键词
水稻/恢复系/稻瘟病/抗性基因/分子标记辅助育种/抗性评价Key words
rice/restorer line/rice blast/resistance genes/molecular marker-assisted breeding/resistance evaluation分类
农业科技引用本文复制引用
孙富,唐梅,卢宏琮,何聪,廖张波..抗稻瘟病水稻恢复系的分子标记辅助选育及抗性鉴定[J].南方农业学报,2024,55(4):1070-1078,9.基金项目
广西科技基地和人才专项(桂科AD20159088) （桂科AD20159088）
广西特色作物试验站"十四五"建设项目(TS202121) （TS202121）
广西农业科学院科技发展基金项目(桂农科2022JM24) （桂农科2022JM24）
广西农业科学院基本科研业务专项(桂农科2021YT034) Guangxi Science and Technology Base and Talent Project(Guike AD2015 9088) （桂农科2021YT034）
Guangxi Charac-teristic Crop Experimental Station"14th Five-Year Plan"Construction Project(TS202121) （TS202121）
Scien-ce and Technology De-velopment Project of Guangxi Academy of Agricultural Sciences(Guinongke 2022JM24) （Guinongke 2022JM24）
Basic Scientific and Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT034) （Guinongke 2021YT034）
