中北大学学报（自然科学版）2024，Vol.45Issue(4)：522-530,9.DOI:10.3969/j.issn.1673-3193.2024.04.014
贝莱斯芽孢杆菌2L-1对两种芝麻病害的生防作用及机理研究
The Biocontrol Effect and Mechanism of Bacillus Velezensis 2L-1 on Two Sesame Diseases
摘要
Abstract
The antibacterial activity of the fermentation supernatant of Bacillus velezensis 2L-1 against plant pathogenic fungi was determined through toxic medium method.The control effect of strain 2L-1 on sesame stem rot and sesame Corynespora leaf spot,as well as its growth-promoting effect on four crops,were determined through pot experiments.To clarify the biocontrol effect and mechanism of strain 2L-1,the enzyme activity during its antagonistic process and its growth-promoting characteristics were tested.Whole genome sequencing and prediction of secondary metabolite gene clusters were performed on strain 2L-1.The results showed that in the toxic medium method,the inhibitory rates of ten times dilution of the fermentation supernatant on pathogens of the sesame stem blight and the sesame Corynespora leaf spot were 91.51%and 83.78%,respectively.This strain can secrete cellulase,protease,and xylanase,and has the characteristics of dissolving phosphorus and producing siderophores.No activities of glucanase and chitinase was detected in the dish.In the potted experiment,the control effect of this strain on sesame seedlings was 45.37%when it was inoculated before Macrophora phaseolina,while the control effect was 29.71%when Macrophora phaseolina was inoculated before this strain on sesame seedlings;When the strain inoculates sesame seedlings before Corynespora cassiicola,the control effect was 25.82%,while when Corynespora cassiicola inoculates sesame seedlings before the strain,the control effect was 33.64%.The 1×108 cfu/mL bacterial suspension of strain 2L-1 had a significant promoting effect on the plant height,fresh weight,and dry weight of the tested crops.The plant height of the tested crops increased by 26.52%to 36.26%,the fresh weight of the aboveground parts increased by 71.43%to 113.64%,and the dry weight increased by 50.00%to 128.57%.Twelve secondary metabolite gene clus-ters were predicted in the genome of this strain.It can be seen that strain 2L-1 has potential applications in sesame diseases prevention and growth promotion.关键词
芝麻茎点枯病/芝麻棒孢叶斑病/贝莱斯芽孢杆菌/生防作用/促生作用Key words
sesame stem rot/sesame Corynespora leaf spot/Bacillus velezensis/biological control mechanisms/growth promotion分类
农业科技引用本文复制引用
刘颖,刘清浩,赵新贝,刘红彦,赵辉,郭金春,李蒙蒙..贝莱斯芽孢杆菌2L-1对两种芝麻病害的生防作用及机理研究[J].中北大学学报（自然科学版）,2024,45(4):522-530,9.基金项目
财政部和农业农村部国家现代农业产业技术体系项目(CARS-14)和河南省农科院优秀青年科技基金资助项目(2022YQ04) （CARS-14）
