裂果薯总皂苷对大鼠肝卵圆细胞系WB-F344上皮间质转化的影响OACSTPCD

Objective:To investigate the effect and mechanism of total saponins of Schizocapsa plantaginea Hance(SSPHs)on epithelial mesenchymal transformation(EMT)of rat hepatic oval cells WB-F344 induced by transforming growth factor-β1(TGF-β1).Methods:WB-F344 cells were divided into control group,model group,low-,medium-and high-dose SSPHs groups and PI3K/AKT signaling pathway inhibitor LY294002 group.Except the control group,all the other groups were induced with 10 μg/L TGF-β1 to establish the EMT model.MTT assay and cell scratch assay were used to detect cell proliferation and migration.Reverse transcrip-tion-quantitative PCR(RT-qPCR)and western blotting were used to detect the mRNA and protein expression of E-cadherin,N-cadherin and Vimentin,respectively.The expression of key proteins in PI3K/AKT signaling path-way was detected by western blotting.Results:SSPHs inhibited the proliferation of TGF-β1-induced WB-F344 cells,with a half maximal inhibitory concentration(IC50)value of 3.02 μg/mL at 24 hours.Compared with the control group,the model group showed significantly increased mRNA and protein expression levels of N-cad-herin and Vimentin,significantly decreased mRNA and protein expression of E-cadherin,and significantly in-creased ratio of p-PI3K/PI3K and p-AKT/AKT(all P＜0.05).Compared with the model group,N-cadherin and Vimentin mRNA and protein expression was down-regulated,E-cadherin mRNA and protein expression was up-regulated,and P-PI3K/PI3K and P-AkT/AKT ratios were decreased in the THE SSPHs medium and high-dose groups(all P＜0.05),with results similar to those observed in the LY294002 group(all P＜0.05).Conclusion:SSPHs can inhibit the EMT process in TGF-β1-induced WB-F344 cells,and its mechanism may be related to the inhibition of PI3K/AKT signaling pathway.