广西医科大学学报2024，Vol.41Issue(5)：653-659,7.DOI:10.16190/j.cnki.45-1211/r.2024.05.003
ARPC1B通过Wnt/β-catenin信号通路调节卵巢癌对顺铂的耐药性
ARPC1B regulates cisplatin resistance in ovarian cancer through the Wnt/β-catenin signaling pathway
摘要
Abstract
Objective:To investigate the effect of ARPC1B on cisplatin resistance in ovarian cancer(OC)through the Wnt/β-catenin signaling pathway and its mechanism of action.Methods:The half maximal inhibito-ry concentration(IC50)values of OC cells(SKOV3)and drug-resistant OC cells(SKOV3/DDP cells)to cisplatin and the expression of ARPC1B were compared.Drug-resistant ovarian cancer cell lines with stable silencing of ARPC1B were constructed,and the IC50 values of SKOV3/DDP cells to cisplatin after knockdown of ARPC1B were detected by cell counting kit-8(CCK-8).Clone formation assay,Transwell and scratch assay were per-formed to determine the proliferation and migration capacities of SKOV3/DDP cells,respectively.The apoptosis was measured by flow cytometry,and apoptosis-associated proteins,as well as the protein expression levels of key molecules of the Wnt/β-catenin signaling pathway were tested by western blotting.Results:The drug resis-tance index of SKOV3/DDP cells was＞2,and ARPC1B was highly expressed in SKOV3/DDP cells(P＜0.05).Silencing of ARPC1B decreased the IC50 values of SKOV3/DDP cells to cisplatin,and the proliferation and migra-tion capacities were weakened(P＜0.05).BAX and Cleaved-Caspase 3 proteins,as well as apoptosis rate were significantly increased in the cells of the ARPC1B knockdown group,while Bcl-2,β-catenin,c-myc and cyclin D1 protein expression levels were reduced(P＜0.05).Conclusion:ARPC1B may inhibit the apoptosis of SKOV3/DDP cells through the Wnt/β-catenin signaling pathway,which in turn enhances the SKOV3/DDP cells to cisplat-in.关键词
卵巢癌/ARPC1B/顺铂耐药/Wnt/β-catenin/凋亡Key words
ovarian cancer/ARPC1B/cisplatin resistance/Wnt/β-catenin/apoptosis分类
医药卫生引用本文复制引用
周海琴,莫世恩,黄俊宁,刘婷基,况燕..ARPC1B通过Wnt/β-catenin信号通路调节卵巢癌对顺铂的耐药性[J].广西医科大学学报,2024,41(5):653-659,7.基金项目
国家自然科学基金资助项目(No.82260566) （No.82260566）
区域性高发肿瘤早期防治研究教育部重点实验室自主课题资助项目(No.GKE-ZZ202136) （No.GKE-ZZ202136）
