烯醇化酶在阴道加德纳菌和乳杆菌的表达及其对糖酵解影响的研究OACSTPCD

Objective:To investigate the differential expression of enolase in the membrane and cytoplasm of Gardnerella vaginalis and Lactobacillus strains,and to compare the differences in their glycolytic abilities.Meth-ods:Gardnerella strains were isolated,purified,and identified from vaginal secretions of patients with bacterial vaginosis.Lactobacillus strains were isolated from vaginal secretions of healthy women and cultured in vitrofor a period of time under the same conditions.The residual concentrations of glucose,lactate,pyruvate,and adenos-ine triphosphate(ATP)in the bacterial solution were detected.The membrane and cytoplasmic proteins of Gard-nerella and Lactobacillus were extracted respectively,and the enolase levels in the cytoplasm and membrane were detected by western blotting.Results:The residual glucose content in the culture medium of Gardnerella was decreased significantly,while the residual glucose content in the culture medium of Lactobacillus was rela-tively higher,and there was significant difference between the two groups(P＜0.05);the amount of ATP pro-duced by Gardnerella was significantly higher than that of Lactobacillus(P＜0.05).When enolase antibodies were added to the reaction system,the amount of residual glucose was increased,while the amount of ATP,lac-tate,and pyruvate was decreased.Enolase was present in the cytoplasm of all Gardnerella and Lactobacillus,and the content of enolase in Gardnerella was significantly higher than that in Lactobacillus(P＜0.05).Only some Gardnerella and Lactobacillus strains had enolase on their cell membranes,and the content of enolase on Gard-nerella cell membranes was significantly higher than that on Lactobacillus cell membranes(P＜0.05).Conclu-sion:There is a certain difference in the expression of enolase between Gardnerella vaginalis and Lactobacillus,which to some extent affects the metabolic ability of the two strains.