广西医科大学学报2024，Vol.41Issue(5)：676-680,5.DOI:10.16190/j.cnki.45-1211/r.2024.05.006
烯醇化酶在阴道加德纳菌和乳杆菌的表达及其对糖酵解影响的研究
Expression of enolase in Gardnerella vaginalis and vaginal Lactobacillus and its effect on gly-colysis
摘要
Abstract
Objective:To investigate the differential expression of enolase in the membrane and cytoplasm of Gardnerella vaginalis and Lactobacillus strains,and to compare the differences in their glycolytic abilities.Meth-ods:Gardnerella strains were isolated,purified,and identified from vaginal secretions of patients with bacterial vaginosis.Lactobacillus strains were isolated from vaginal secretions of healthy women and cultured in vitrofor a period of time under the same conditions.The residual concentrations of glucose,lactate,pyruvate,and adenos-ine triphosphate(ATP)in the bacterial solution were detected.The membrane and cytoplasmic proteins of Gard-nerella and Lactobacillus were extracted respectively,and the enolase levels in the cytoplasm and membrane were detected by western blotting.Results:The residual glucose content in the culture medium of Gardnerella was decreased significantly,while the residual glucose content in the culture medium of Lactobacillus was rela-tively higher,and there was significant difference between the two groups(P＜0.05);the amount of ATP pro-duced by Gardnerella was significantly higher than that of Lactobacillus(P＜0.05).When enolase antibodies were added to the reaction system,the amount of residual glucose was increased,while the amount of ATP,lac-tate,and pyruvate was decreased.Enolase was present in the cytoplasm of all Gardnerella and Lactobacillus,and the content of enolase in Gardnerella was significantly higher than that in Lactobacillus(P＜0.05).Only some Gardnerella and Lactobacillus strains had enolase on their cell membranes,and the content of enolase on Gard-nerella cell membranes was significantly higher than that on Lactobacillus cell membranes(P＜0.05).Conclu-sion:There is a certain difference in the expression of enolase between Gardnerella vaginalis and Lactobacillus,which to some extent affects the metabolic ability of the two strains.关键词
细菌性阴道病/烯醇化酶/阴道加德纳菌/阴道乳杆菌/糖酵解Key words
bacterial vaginosis/enolase/Gardnerella vaginalis/vaginal Lactobacillus/glycolysis分类
医药卫生引用本文复制引用
张瑞,吴益青,尹海旭,李昶,马爱昕,王君竹,白会会,刘朝晖,廖秦平..烯醇化酶在阴道加德纳菌和乳杆菌的表达及其对糖酵解影响的研究[J].广西医科大学学报,2024,41(5):676-680,5.基金项目
国家自然科学基金资助项目(No.81471420) （No.81471420）
教育部博士点新教师基金项目(No.20130001120051) （No.20130001120051）
