广西医科大学学报2024，Vol.41Issue(5)：681-687,7.DOI:10.16190/j.cnki.45-1211/r.2024.05.007
红景天苷调控lncRNA HOTAIR/NF-κB促进脊髓损伤后运动功能机制研究
Study on the mechanism of salidroside regulating lncRNA HOTAIR/NF-κB to promote motor function after spinal cord injury
摘要
Abstract
Objective:To investigate the effect of salidroside(SAL)regulating long non-coding RNA HOX tran-script antisense RNA(lncRNA HOTAIR)on motor function after spinal cord injury(SCI).Methods:Twenty-sev-en SD rats were randomly divided into sham surgery group,SCI group,and SCI+SAL group.A rat model of SCI was established using the modified Allen's method.BBB scoring was used to assess the motor function of the rats'hind limbs.Hematoxylin-eosin(HE)staining and Nissl staining were used to observe the spinal cord tissue structure and the number of neurons.The expression of HOTAIRand related inflammatory factors was deter-mined by reverse transcription-quantitative PCR(RT-qPCR).The lncRNA HOTAIR overexpressed astrocytes were cultured in vitro,and then divided into 8 groups along with primary astrocytes:control group and SAL group with different concentrations(6.25 μg/mL,12.5 μg/mL,25 μg/mL,50 μg/mL,100 μg/mL,200 μg/mL and 400 μg/mL).The cell survival rate was assessed using the cell counting kit-8(CCK-8)method.The model of sec-ondary SCI inflammation was established using Lipopolysaccharide(LPS),and SAL was administered at varying concentrations.The gene expression of lncRNA HOTAIR,IL-6,TNF-α was detected by RT-qPCR,and the pro-tein expression of NF-κB pathway and inflammatory factors was determined by western blotting and enzyme-linked immunosorbent assay(ELISA),respectively.Results:The intervention of SAL significantly enhanced the BBB score in SCI rats,mitigated tissue structure destruction and neuronal damage,and down-regulated the ex-pression of HOTAIRand inflammatory factors(TNF-α,IL-6)following SCI.In vitrostudies revealed no signifi-cant alteration in astrocyte survival rates across different doses groups of SAL compared with the control group.Further investigations demonstrated that SAL could modulate lncRNA HOTAIR to reduce the expression of in-flammatory factors in LPS-stimulated astrocytes,while also targeting lncRNA HOTAIR to down-regulate the ex-pression of p-NF-κB p65,p-IκB-α,IKKβ proteins and inflammatory factors.Conclusion:SAL can promote the recovery of motor function in SCI rats,and its mechanism may be related to reducing inflammation and inhibit-ing the expression of lncRNA HOTAIR.关键词
红景天苷/HOX转录反义RNA/炎症/脊髓损伤Key words
salidroside/HOX transcript antisense RNA/inflammation/spinal cord injury分类
医药卫生引用本文复制引用
谭秀伟,巫艳岚,李俸鑫,卢军良,杨佰辉,苏义基..红景天苷调控lncRNA HOTAIR/NF-κB促进脊髓损伤后运动功能机制研究[J].广西医科大学学报,2024,41(5):681-687,7.基金项目
国家自然科学基金资助项目(No.81960773) （No.81960773）
广西自然科学基金资助项目(No.2023GXNSFAA026149) （No.2023GXNSFAA026149）
广西医科大学"四新"立项项目(No.SX202228) （No.SX202228）
广西研究生教育创新计划资助项目(No.JGY2022075) （No.JGY2022075）
