广西医科大学学报2024，Vol.41Issue(5)：688-696,9.DOI:10.16190/j.cnki.45-1211/r.2024.05.008
铜代谢相关基因COX17与头颈部鳞状细胞癌不良预后相关
The copper metabolism-related gene COX17 is associated with poor prognosis of head and neck squamous cell carcinoma
摘要
Abstract
Objective:To investigate the impact of copper metabolism-related gene cytochrome c oxidase copper chaperone 17(COX17)on the occurrence,development and prognosis of head and neck squamous cell carcino-ma(HNSCC).Methods:Bioinformatics analysis was employed to assess the expression differences of COX17 between HNSCC and normal tissues.Nomogram was generated to verify the role of COX17in predicting the prognosis of HNSCC.Functional enrichment analysis of COX17-related genes was conducted using KEGG and GO analyses,and the relationship between COX17expression and the abundance immune cell infiltration in HN-SCC tissues was analyzed using ssGSEA.Reverse transcription-quantitative polymerase chain reaction(RT-qP-CR)was utilized to verify the expression differences of COX17between HNSCC cell lines and immortalized nor-mal epithelial cell lines.The effect of COX17on cell proliferation and invasion was assessed using the cell count-ing kit-8(CCK-8)assay and Transwell experiments,respectively.Results:COX17expression was significantly up-regulated in HNSCC(P＜0.05).Prognostic analysis indicated that COX17expression was closely associated with the overall survival(OS)of HNSCC patients(P＜0.05).Immune infiltration correlation analysis revealed a significant negative correlation between COX17expression and the abundance of various immune cell infiltration(all P＜0.001).Knocking down COX17significantly inhibited the proliferation and invasion abilities of HNSCC cell line SCC-9 and SAS.Conclusion:The expression of COX17can affect the proliferation and invasion of HN-SCC cells and is potentially associated with immune cell infiltration in HNSCC tissues.As a biomarker of mito-chondrial copper metabolism,COX17may be an effective indicator for assessing the prognosis of HNSCC and a potential therapeutic target.关键词
细胞色素c氧化酶铜伴侣17/铜代谢/头颈鳞癌/生长/侵袭/预后Key words
cytochrome c oxidase copper chaperone 17/copper metabolism/head and neck squamous cell carci-noma/growth/invasion/prognosis分类
医药卫生引用本文复制引用
卢苇,江川,何馨露,姚茜,苏敬雅,刘春磊,谢莹,韦正波..铜代谢相关基因COX17与头颈部鳞状细胞癌不良预后相关[J].广西医科大学学报,2024,41(5):688-696,9.基金项目
国家自然科学基金资助项目(No.82160386) （No.82160386）
广西自然科学基金资助项目(No.2021GXNSFAA075042 （）
No.2023GXNSFAA026189) （）
